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首页> 外文期刊>Coatings >Biomimetic Properties of Force-Spun PHBV Membranes Functionalised with Collagen as Substrates for Biomedical Application
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Biomimetic Properties of Force-Spun PHBV Membranes Functionalised with Collagen as Substrates for Biomedical Application

机译：用胶原蛋白酶官能化的肌肉纺布膜作为生物医学应用的底物

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The force-spinning process parameters (i.e., spin speed, spinneret-collector distance, and polymer concentration), optimised and characterised in previous work by this group, allowed the rapid fabrication of large quantities of high surface area poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) polymeric fibre membranes. This paper examined the potential application for force-spun PHBV fibres functionalised with type I collagen for tissue regeneration applications. PHBV fibre scaffolds provide a biologically suitable substrate to guide the regeneration of dermal tissues, however, have poor cellular adhesion properties. The grafting of collagen type-I to PHBV fibres demonstrated improved cell adhesion and growth in Neo-NHDF (neonatal human dermal fibroblasts) fibroblasts. The examination of fibre morphology, thermal properties, collagen content, and degradability was used to contrast the physicochemical properties of the PHBV and PHBV-Collagen fibres. Biodegradation models using phosphate buffered saline determined there was no appreciable change in mass over the course of 6 weeks; a Sirius Red assay was performed on degraded samples, showing no change in the quantity of collagen. Cell metabolism studies showed an increase in cell metabolism on conjugated samples after three and 7 days. In addition, in vitro cytocompatibility studies demonstrated superior cell activity and adhesion on conjugated samples over 7 days.
机译：在此组的先前工作中优化和特征在于，纺丝工艺参数（即，旋转速度，喷丝器集电极距离和聚合物浓度）允许快速制造大量的高表面积聚（3-羟基丁酸 - CO-3-羟基甲酸）（PHBV）聚合物纤维膜。本文研究了用I型胶原蛋白官能化的力 - 纺PHBV纤维的潜在应用，用于组织再生应用。 PHBV纤维支架提供生物合适的基材，以引导真皮组织的再生，然而，细胞粘附性能差。胶原蛋白-I至PHBV纤维的接枝显示出新的细胞粘附和新生儿（新生儿人皮肤成纤维细胞）成纤维细胞的增长。用于对纤维形态，热性能，胶原甲烯含量和可降解性的检查用于对比PHBV和PHBV-胶原纤维的物理化学性质。使用磷酸盐缓冲盐水的生物降解模型确定在6周内没有明显的质量变化;在降解的样品上进行Sirius红色测定，显示出胶原蛋白的数量没有变化。细胞代谢研究表明，在三次和7天后缀合样品上的细胞代谢增加。此外，体外的细胞膜相容性研究在7天内显示出优异的细胞活性和缀合的样品上的粘附。

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