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首页> 外文期刊>Contemporary Clinical Dentistry >Osteogenic differentiation of human amniotic mesenchymal stem cells in chitosan-carbonate apatite scaffold (in vivo study)
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Osteogenic differentiation of human amniotic mesenchymal stem cells in chitosan-carbonate apatite scaffold (in vivo study)

机译：壳聚糖 - 碳酸盐磷灰石支架中的人羊膜间充质干细胞（体内研究中的骨质化分化

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Background: Studies of bone tissue engineering as a viable alternative to autogenous bone graft show promising results, although its mechanism and effectiveness remain only partially understood. Purpose: to explain the osteogenic differentiation of scaffold chitosan (Ch)–carbonate apatite (CA) in seeding with human amniotic mesenchymal stem cells (hAMSCs) on the regeneration of calvarial bone defects in rats. Materials and Methods: Shitosan-Carbonate Apatite (Ch-CA) scaffold was created by means of a freeze-drying method. Twenty Wistar rats were randomly divided into two groups: control and treatment. Defects were created in the calvarial bone of each treatment group with a scaffold subsequently implanted. After 8 weeks, the rats were terminated for histology and immunohistochemistry examination. Results: Expressions of vascular endothelial growth factor, bone morphogenetic protein2, Runt-related transcription factor 2 (RUNX2), and angiogenesis occurred earlier in the tissue-engineered group than that in the control group. An 8-week analysis also showed that the expression of RUNX2, alkaline phosphatase, osteocalcin, and collagen type 1 was at more elevated levels in the treatment group than that in the control group. Conclusion: These results showed that the combination of hAMSCs and Ch-CA scaffold may become one of the candidates for bone tissue engineering.
机译：背景：骨组织工程作为自生骨移植物的可行替代方案的研究表明，虽然其机制和有效性仍然是部分地理解的。目的：解释用人羊膜间充质干细胞（HAMSCs）在大鼠颅骨骨缺陷再生中播种碎屑壳聚糖（CH） - 碳酸盐（CH） - 碳酸盐（CO）的成骨分化。材料和方法：通过冷冻干燥方法产生Shitosan-碳酸盐磷灰石（CH-CA）支架。将二十个Wistar大鼠随机分为两组：控制和治疗。用随后植入的支架在每个治疗组的颅骨中产生缺陷。 8周后，大鼠终止组织学和免疫组织化学检查。结果：血管内皮生长因子，骨形态发生蛋白2，runt相关转录因子2（RUNX2）的表达，以及组织工程群中的血管生成在对照组中发生。 8周的分析还显示Runx2，碱性磷酸酶，骨钙蛋白和胶原蛋白1的表达在治疗组中的水平更高，比对照组更高。结论：这些结果表明，Hamscs和Ch-CA支架的组合可能成为骨组织工程的候选者之一。

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