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首页> 外文期刊>Current Journal of Applied Science and Technology >Molecular Detection of Fusarium oxysporum f. sp. Cubense Race TR4 Infecting Banana Groups in Indo-Gangetic Plains of India
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Molecular Detection of Fusarium oxysporum f. sp. Cubense Race TR4 Infecting Banana Groups in Indo-Gangetic Plains of India

机译：镰刀镰刀菌的分子检测。 sp。 Cumense Race TR4感染印度印度印度印度平原的香蕉群体

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摘要

Panama wilt or Fusarium wilt incited by Fusarium oxysporum f. sp. cubense (Foc) is a major threat for banana cultivation and impacting symbolic economic losses worldwide. Previously Foc race 1 and race 2 was detected in India which eventually spread monoculture of ‘Cavendish’ bananas resistant against these two races. Later on, another new race TR4 of Foc was detected to be incited in ‘Cavendish’ group of banana raised an alarming situation to the global banana industry. In the present study detailed survey was conducted in popular banana growing districts of West Bengal, India for visualizing the impact of disease incidence as well as collection of isolates from infected suckers and rhizomes of ‘Cavendish’ banana with an aim to develop a rapid detection technique for apprehending the presence of Foc TR4 race at early infection stages. Methods comprised of field survey followed by isolation of pathogen, test of pathogenicity and further molecular detection through Foc TR4 specific primers. Results revealed that the colonies formed on the potato dextrose agar (PDA) medium exhibited typical characteristic of Foc. Pathogenicity test conducted by considering healthy plantlet of ‘Grand Naine’ also detected characteristic symptom of Foc. Additionally, PCR assay using specific markers followed by sequencing of 28S-18S ribosomal RNA intergenic spacer spacer (IGS) confirmed the presence of this isolate in the infected samples. Phylogenetic evolution assays revealed a very close relationship of the Foc TR4 strains of India with Asia-Pacific isolates of Foc TR4. This study advocated the urgency of prevention of introduction of Foc TR4 into disease-free areas in the banana growing districts of India for maintaining sustainable banana production.
机译：巴拿马威尔特或镰刀菌苏黎苏氏菌苏黎氏菌。 sp。 Cumense（Foc）是对香蕉种植和影响象征性经济损失的主要威胁。此前在印度检测到Foc Race 1和Race 2，最终将“Cavendish”香蕉的单一栽培造成抵抗这两场比赛。后来，在“Cavendish”的香蕉群中，另一个新的Foc的新比赛TR4被招募了一个令人震惊的局面，以至于全球香蕉行业。在目前的研究中，在印度西孟加拉邦的流行香蕉种植区进行了详细的调查，用于可视化疾病发病率的影响以及感染者的吸盘和“卡文普”香蕉的孤立株的孤立株的影响，目的是开发快速检测技术为了在早期感染阶段逮捕Foc TR4种族的存在。由现场调查组成的方法，然后分离病原体，致病性试验，通过FoC TR4特异性引物进行致病性和进一步的分子检测。结果表明，在马铃薯右旋糖琼脂（PDA）培养基上形成的菌落表现出Foc的典型特征。通过考虑“大奈内”的健康植物进行的致病性试验检测到Foc的特征症状。另外，使用特异性标记的PCR测定随后进行28秒-18S核糖体RNA代骨间隔物间隔物（IGS）证实了在感染的样品中存在该分离物的存在。系统发育演化测定揭示了印度的Foc Tr4菌株与亚太地区的FOC TR4的亚太区分离株非常密切。这项研究主张预防印度香蕉种植区的无疾病区内的疾病区的紧迫性，以维持可持续的香蕉生产。

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