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首页> 外文期刊>ACS Omega >Naturally Occurring Phenols Modulate Vegetative Growth and Deoxynivalenol Biosynthesis in Fusarium graminearum
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Naturally Occurring Phenols Modulate Vegetative Growth and Deoxynivalenol Biosynthesis in Fusarium graminearum

机译：天然存在的酚在镰刀酸纤维素中调节营养生长和脱氧肾上腺素生物合成

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摘要

To assess the in vitro activity of five naturally occurring phenolic compounds (ferulic acid, apocynin, magnolol, honokiol, and thymol) on mycelial growth and type B trichothecene mycotoxin accumulation by Fusarium graminearum , three complementary approaches were adopted. First, a high-throughput photometric continuous reading array allowed a parallel quantification of F. graminearum hyphal growth and reporter TRI5 gene expression directly on solid medium. Second, RT-qPCR confirmed the regulation of TRI5 expression by the tested compounds. Third, liquid chromatography–tandem mass spectrometry analysis allowed quantification of deoxynivalenol (DON) and its acetylated forms released upon treatment with the phenolic compounds. Altogether, the results confirmed the activity of thymol and an equimolar mixture of thymol–magnolol at 0.5 mM, respectively, in inhibiting DON production without affecting vegetative growth. The medium pH buffering capacity after 72–96 h of incubation is proposed as a further element to highlight compounds displaying trichothecene inhibitory capacity with no significant fungicidal effect.
机译：为了评估五种天然存在的酚类化合物（阿魏酸，呼吸皂苷蛋白，镁，蜂鸣器，蜂鸣器和胸腺酚）对菌丝体生长和B型Trichothecene霉菌毒素积累的体外活性，采用了三种互补方法。首先，允许高通量的光度连续读数阵列允许 f的并联定量。直接在固体培养基上直接在固体培养基上的克柳素悬垂生长和报告者 TRI5基因表达。其次，RT-QPCR通过测试的化合物证实了三5表达的调节。第三，液相色谱 - 串联质谱分析允许定量脱氧苯酚（Don）及其用酚类化合物处理后释放的乙酰化形式。总共，结果证实了百里酚和粒醇 - 镁醇的等摩尔混合物的活性分别在0.5mm处抑制唐生产而不影响营养生长。提出培养72-96小时后的培养基缓冲能力作为进一步的元素，以突出显示呈现三胞素抑制能力的化合物，没有显着的杀菌效果。

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