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Integrated Analysis of Transcriptome and Metabolome in Cirsium japonicum Fisch ex DC

机译：Cirsium japonicum FISCH EX DC中转录组和代谢物的综合分析

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Cirsium japonicum Fisch ex DC belongs to the Compositae family and has been used as a folk remedy source in Asian countries because of its health-promoting properties. It is known that C. japonicum contains flavonoids, furans, long-chain alcohols, sterols, and volatile oils. Nevertheless, the molecular mechanism of secondary metabolite biosynthesis remains poorly understood. Therefore, transcriptome analysis and metabolic profiling were performed using different parts of C. japonicum to investigate phenylpropanoid metabolism. Based on the BLASTX search results, we identified 29 orthologs of enzymes responsible for phenylpropanoid biosynthesis. Additionally, 75 metabolites were identified in C. japonicum . Most of the flavonoid biosynthetic genes were significantly expressed ranging from 2.6- to 500-fold higher in the flowers than those in the leaves. Correspondently, the total content of flavonols was 21-fold higher in the flowers than in the roots. However, the total level of flavones showed 58-fold higher amounts in the leaves than in the flowers. Additionally, the total content of flavanols was 19-fold higher in the leaves than in the roots. The results of this study provide transcriptomic and metabolic information to elucidate the tissue-specific phenylpropanoid metabolism of C. japonicum .
机译：柳枝茎FISCH EX DC属于Compositae系列，由于其健康的财产，已被用作亚洲国家的民间补救措施。众所周知， c。日本含有黄酮类化合物，呋喃，长链醇，甾醇和挥发性油。然而，次级代谢产物生物合成的分子机制仍然明显差不多。因此，使用不同部分的 C进行转录组分析和代谢分析。粳稻研究苯丙醇代谢。基于BLASTX搜索结果，我们确定了29种酶，其负责苯丙烷化生物合成。另外，在 c中鉴定了75个代谢物。 japonicum。大多数黄酮类生物合成基因显着表达在花中比叶子中的比例更高的2.6-至500倍。相应地，黄酮醇的总含量在花中比根部更高21倍。然而，黄酮的总水平显示在叶子中比在花中的较高量增加58倍。另外，黄黄烷醇的总含量比根状物高19倍。该研究的结果提供转录组和代谢信息，以阐明 C的组织特异性苯丙醇代谢。 japonicum。

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