...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>ACS Omega >Process Development for 6-Aminopenicillanic Acid Production Using Lentikats-Encapsulated Escherichia coli Cells Expressing Penicillin V Acylase
【24h】

Process Development for 6-Aminopenicillanic Acid Production Using Lentikats-Encapsulated Escherichia coli Cells Expressing Penicillin V Acylase

机译：使用锂封装的大肠杆菌细胞的6-氨基丙酸酸生产的过程开发用脑青霉素V acyl酶

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Penicillin V acylase (PVA, EC 3.5.1.11) hydrolyzes the side chain of phenoxymethylpenicillin (Pen V) and finds application in the manufacture of the pharmaceutical intermediate 6-aminopenicillanic acid (6-APA). Here, we report the scale-up of cultivation of Escherichia coli whole cells expressing a highly active PVA from Pectobacterium atrosepticum and their encapsulation in polyvinyl alcohol–poly(ethylene glycol) Lentikats hydrogels. A biocatalytic process for the hydrolysis of 2% (w/v) Pen V was set up in a 2 L reactor using the Lentikats-immobilized whole cells, with a customized setup to enable continuous downstream processing of the reaction products. The biocatalytic reaction afforded complete conversion of Pen V for 10 reaction cycles, with an overall 90% conversion up to 50 cycles. The bioprocess was further scaled up to the pilot-scale at 10 L, enabling complete conversion of Pen V to 6-APA for 10 cycles. The 6-APA and phenoxy acetic acid products were recovered from downstream processing with isolated yields of 85–90 and 87–92%, respectively. Immobilization in Lentikats beads improved the stability of the whole cells on storage, maintaining 90–100% activity and similar conversion efficiency after 3 months at 4 °C. The robust PVA biocatalyst can be employed in a continuous process to provide a sustainable route for bulk 6-APA production from Pen V.
机译：青霉素V acylase（PVA，EC 3.5.111）水解苯氧基甲基百年霉素（笔V）的侧链，并在制备药物中间体6-氨基喹硫甲酸（6-APA）中的应用。在这里，我们报告了表达高活性PVA的大肠杆菌的培养的扩大率，从胶质杆菌患者和它们在聚乙烯醇 - 聚（乙二醇）Lentikats水凝胶中的包封。使用锂固定的全细胞在2L反应器中设立了2％（w / v）笔V的生物催化方法，使用定制设置，以实现反应产物的连续下游加工。生物催化反应得到了对10个反应循环的笔v完全转化，总共90％转化率为50次循环。进一步将BioProcess进一步缩放到10L的试验尺度，使笔V至6-APA的完全转化为10个循环。 6-APA和苯氧基乙酸产物分别从下游加工中回收，分离出85-90％和87-92％。熊熊湖珠子的固定化改善了整个细胞对储存的稳定性，在4℃下3个月后保持90-100％的活性和类似的转化效率。鲁棒PVA生物催化剂可以在连续过程中使用，以提供从笔V的批量6-APA生产的可持续路线。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号