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首页> 外文期刊>African Journal of Biotechnology >Effects of adenine sulphate, glutamine and casein hydrolysate on in vitro shoot multiplication and rooting of Kinnow mandarin (Citrus reticulata Blanco)
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Effects of adenine sulphate, glutamine and casein hydrolysate on in vitro shoot multiplication and rooting of Kinnow mandarin (Citrus reticulata Blanco)

机译：硫酸盐，谷氨酰胺和酪蛋白水解产物对体外拍摄倍增和生根的影响（柑橘reticulata blanco）

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In vitro?shoot regeneration was carried out for Kinnow mandarin (Citrus reticulataBlanco) through shoot tip explants obtained from?in vitro?germinated seedling. The medium supplemented with 2.5 mg/L benzylaminopurine (BAP) supported maximum shoot proliferation (2.45 shoots/explant). This rate was further enhanced (7.23 shoots/explant) by adding 25 mg/L glutamine, 50 mg/L adenine sulphate and 100 mg/L casein hydrolysate. From the?in vitro?proliferated shoots, the shoot apices and nodes were excised and sub-cultured on four different medium namely: SCM-1, SCM-2, SCM-3 and SCM-4 for six consecutive months. Problems like defoliation, chlorosis and loss of vigour in shoots were observed at different stages of subculture. These problems were successfully controlled by SCM-4 having 1.5 mg/L of BAP, 0.5% charcoal, 25 mg/L glutamine, 50 mg/L adenine sulphate and 100 mg/L casein hydrolysate. The SCM-4 also maintained the high proliferation rate throughout the subculturing process. Rooting was best induced on 2 mg/L indole-butyric acid (IBA) in combination with 0.1 mg/L indole-3-acetic acid (IAA). Rooting was significantly enhanced by 100 mg/L casein hydrolysate. The?in vitro?raised plantlets were acclimatized under culture room conditions in different potting mixture, of which the combination of garden soil, sand and vermiculite mixture in 1:1:2 ratio was found most supportive. After 30 days of acclimatization, plantlets were transferred to soil, where established plants showed more than 90% survival.
机译：体外α芽再生用于Kinnow普通话（柑橘reticulatablanco）通过射击尖端外植体进行，从体外培养植体进行发芽的幼苗。补充有2.5mg / L苄基氨基嘌呤（BAP）的培养基支持最大次芽增殖（2.45芽/勘探型）。通过加入25mg / l谷氨酰胺，50mg / L腺嘌呤硫酸盐和100mg / L柴蛋白水解产物，进一步增强了（7.23芽/脱钙）。从体外α增殖芽，切除射击和节点在四种不同的介质上被切除并亚培养：SCM-1，SCM-2，SCM-3和SCM-4连续六个月。在脱培养的不同阶段观察到脱落，萎缩和枝条活力丧失的问题。这些问题被SCM-4成功控制，具有1.5mg / L的BAP，0.5％木炭，25mg / L谷氨酰胺，50mg / L腺嘌呤硫酸盐和100mg / L柴油水解产物。 SCM-4在整个传代培养过程中也保持了高增殖率。生根最好诱导2mg / L吲哚-丁酸（IBA）与0.1mg / L吲哚-3-乙酸（IAA）组合。通过100mg / L酪蛋白水解产物显着增强生根。体外培养的小植物在不同灌封混合物中的培养室条件下适应，其中园林土壤，砂和蛭石混合物的组合在1：1：2的比例中得到了最多的基础。 30天内适应30天后，将Plantlet转移到土壤中，成立的植物的存活率超过90％。

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