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首页> 外文期刊>African Journal of Biotechnology >Screening of in vitro derived mutants of banana against nematodes
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Screening of in vitro derived mutants of banana against nematodes

机译：对线虫香蕉体外衍生突变体的筛选

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Investigations were carried out to screen the in vitro derived mutants of banana cv. Robusta (Caveidish- AAA) and Rasthali (Silk- AAB) by using certain bio-chemical parameters including some enzyme activities. The mutants tested were Ro Im V4 6-1-1, Ro Im V4 6-1-2, Ro Im V4 6-2-1, Si Im V4 10-5-3, Si Im V4 6-2-5 along with respective susceptible checks (Robusta and Rasthali), tolerant check (Anaikomban- AA) and resistant check (Pisang Lilin- AA). Various biochemical assays used were total phenol, tannin content, lignin content, peroxidase, polyphenol oxidase, phenyl alanine ammonia lyase and ascorbic acid oxidase. The results revealed that the mutants namely Ro Im V4 6-1-1 and Si Im V4 10-5-3 were found to be resistant while the mutant Ro Im V4 6-2-1 was moderately resistant. The rest of the mutants namely Ro Im V4 6-1-2 and Si Im V4 6-2-5 were found to be susceptible to nematodes. The resistant and moderately resistant mutants of banana could be further used in breeding programmes as well as being recognized as potential cultivars of commerce.
机译：进行研究以筛选香蕉CV的体外衍生突变体。通过使用包括一些酶活性的某些生物化学参数，Robusta（Caveidish-AAA）和Rasthali（丝绸）。测试的突变体是RO IM V4 6-1-1，RO IM V4 6-1--，RO IM V4 6-2-1，SI IM V4 10-5-3，SI IM V4 6-2-5以及各自的易感检查（Robusta和Rasthali），耐受检查（AnaiCombanan-AA）和抗性检查（Pisang Lilin-AA）。所用的各种生化测定是总苯酚，单宁含量，木质素含量，过氧化物酶，多酚氧化酶，苯基丙氨酸氨酶和抗坏血酸氧化酶。结果显示，发现突变体是RO IM V4 6-1-1和Si IM V4 10-5-3在突变体RO IM V4 6-2-1中抗性。发现其余的突变体即RO IM V4 6-1-2和Si IM V4 6-2-5易于线虫。香蕉的抗性和中度抗性突变体可以进一步用于育种计划以及被认为是商业的潜在品种。

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