Cry3A -endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop

Josef VLASáK; Jind?ich B?íZA; Daniela PAVINGEROVá; Roman MODLINGER; Milo? KNí?EK; Jana MALá

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Cry3A -endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop

机译：cry3a-诱变毒素的毒素，以增强毒性，在受体结合环中溢出吠毛甲虫

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Bacillus thuringiensis Cry3A?gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed inEscherichia coli?BL21 and their toxicity on spruce bark beetle larvae was tested using spruce bark sandwiches. Mutant toxins with N-terminal deletion and loop 1 duplication showed increased toxicity.

机译：Bacillus thuringiensis Cry3a？基因重新设计用于挪威云杉的高表达，略微修饰序列，以允许简单的N-和C-末端缺失和结构域II环1换合合成寡核苷酸。从合成基因的13种变体中的修饰Cry3a毒素表达了Inescherichia Coli？BL21，并使用云杉树皮三明治测试了对云杉树皮甲虫幼虫的毒性。具有N-末端缺失和环1重复的突变毒素显示出增加的毒性。

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《African Journal of Biotechnology》 |2012年第86期|共5页
作者
Josef VLASáK; Jind?ich B?íZA; Daniela PAVINGEROVá; Roman MODLINGER; Milo? KNí?EK; Jana MALá;
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Bacillus thuringiensisIps typographusPicea abiesresistance;

机译：Bacillus thuringiensisips typographuspicea abiesyistance;

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1. Enhanced toxicity of Bacillus thuringiensis Cry3A δ‐endotoxin in coleopterans by mutagenesis in a receptor binding loop [J] . Bauer Leah S., Dean Donald H., Koller C.Noah, FEBS Letters . 2000,第2期

机译：通过受体结合环中的诱变增强苏云金芽孢杆菌Cry3Aδ-内毒素对鞘翅目的毒性
2. Occurrence of the European spruce bark beetle Ips typographus (L.) and six-toothed spruce bark beetle Pityogenes chalcographus (L.) in spruce stands damaged by wind in the ?wi?tokrzyskie Mountains [J] . Andrzej Borkowski Forest Research Papers . 2011,第1期

机译：欧洲云杉树皮甲虫Ips typographus（L.）和云杉林中的六齿云杉树皮甲虫Pityogenes chalcographus（L.）发生在？wi？tokrzyskie山脉被风破坏
3. Description of Bursaphelenchus piceae sp n. (Nematoda: Parasitaphelenchinae) from larval galleries of the six-toothed spruce bark beetle, Pityogenes chalcographus (L.) (Coleoptera: Curculionidae: Scolytinae), in Norway spruce, Picea abies (L.) Karsten [J] . Tomalak Marek, Pomorski Jan J. Nematology . 2015,第10期

机译：Bursaphelenchus piceae sp n。的描述（Nematoda：Parasitaphelenchinae）产自挪威的云杉，云杉云杉（Pice abies（L.））的六齿云杉树皮甲虫Pityogenes chalcographus（L.）
4. Normalized Projected Red SWIR (NPRS): A New Vegetation Index for Forest Health Estimation and Its Application on Spruce Bark Beetle Attack Detection [C] . Langning Huo, Eva Lindberg, Henrik Persson International Geoscience and Remote Sensing Symposium . 2020

机译：规范化预计的红色和苏格兰州（NPRS）：森林健康估算的新植被指标及其对云杉甲虫虫攻击检测的应用
5. Engelmann Spruce Survival and Regeneration After an Epidemic Spruce Beetle Outbreak on the Markagunt Plateau in Southern Utah [D] . Pettit, Jessika M. 2018

机译：Engelmann在犹他州南部的Markagunt高原疫情爆发后狂潮云杉的生存和再生
6. A Common Fungal Associate of the Spruce Bark Beetle Metabolizes the Stilbene Defenses of Norway Spruce [O] . Almuth Hammerbacher, Axel Schmidt, Namita Wadke, 2013

机译：云杉的树皮甲虫的普通真菌同伴代谢挪威云杉的天敌防御物
7. Enhanced toxicity of Bacillus thuringiensis Cry3A δ-endotoxin in coleopterans by mutagenesis in a receptor binding loop [O] . Wu Sheng-Jiun, Koller C.Noah, Miller Deborah L., 2000

机译：受体结合环中的诱变提高了苏云金芽孢杆菌Cry3Aδ-内毒素对鞘翅目的毒性

Cry3A -endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop

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