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In vitro regeneration, flowering, and cell culture of Centaurea species

机译:体外再生,开花和细胞培养物种

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This study was conducted to establish a protocol for?in vitro?flowering of?Centaurea cyanus?and cell cultures of?Centaurea montana. In four weeks, 50 to 60 adventitious shoots developed on leaf explants cultured in MS medium supplemented with 2.0 mg/L benzylaminopurine (BAP) and 0.1 mg/L indole-3-acetic acid (IAA).?In vitroflowering and seed set occurred in?C. cyanus?when the shoots were incubated on MS basal medium supplemented with B5 vitamins, 100 mg/L myo-inositol and 30 g/L sucrose for 4 weeks under 16 h photoperiod. Young leaves of?C. montana?cultured on MS medium supplemented with 1.0 to 6.0 mg/L 2, 4- dichloroxypenoxyacetic acid (2, 4-D) alone or in combination with 0.5 mg/L BAP generated callus. Liquid MS medium containing 2.0 mg/L 2, 4-D produced greater fresh weight (FW), dry weight (DW) and packed cell volume (PCV) compared to MS medium with 1 mg/L 2, 4-D in a 30 day culture cycle. The results indicate that MS medium modified with appropriate phytohormones can be used to achieve efficient shoot regeneration and?in vitroflowering of?C. cyanus?and cell cultures of?C. montana.
机译:进行了该研究以建立一个议定书的α-α-·塞纳西亚植物的开花?和Centaurea Montana的细胞培养物。在四周内,50至60个不定芽在培养的MS培养体中培养的叶子外植物,其补充有2.0mg / L苄氨基嘌呤(BAP)和0.1mg / L吲哚-3-乙酸(IAA)。?在vitroflowering和种子集中发生?C。 Cyanus?当芽孵育在补充有B5维生素,100mg / L肌醇和30g / L蔗糖的MS基础培养基上,在16小时光周期下4周。年轻的叶子?c。蒙大拿州?在单独或与0.5mg / L Bap产生的愈伤组织组合的MS培养物培养1.0至6.0mg / L 2,4-二氯酰氧杂酸(2,4-D)。与MS培养基相比,含有2.0mg / L 2,4-D的液体MS培养基,其含有1mg / L 2,4-D的MS培养基,在30℃下产生更高的新鲜重量(FW),干重(DW)和填充细胞体积(PCV)日文化周期。结果表明,用适当的植物激素改性的MS培养基可用于实现有效的芽再生和?在vitroflowering的Δc中。 cyanus?和细胞培养物?c。蒙大拿。

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