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首页> 外文期刊>African Journal of Biotechnology >Effect of lead on metallothionein concentration in lead-resistant bacteria Bacillus cereus isolated from industrial effluent
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Effect of lead on metallothionein concentration in lead-resistant bacteria Bacillus cereus isolated from industrial effluent

机译：铅对工业流出物分离的耐铅菌芽孢杆菌中金属硫蛋白浓度的影响

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摘要

Metallothioneins (MTs) are cysteine-rich metal-binding proteins found in a wide variety of organisms including bacteria, fungi as well as all eukaryotic plant and animal species. MTs bind essential and non-essential heavy metals. MTs production was evaluated by a simple spectrophotometry methodology. The study elucidated that the metallothionein concentration in?Bacillus cereus?treated with different concentrations of lead increased with increasing lead concentration. The protein samples from?B.cereus?treated with different concentrations of lead were isolated and electrophoresed on SDS-PAGE and 2-D gels. The study of gels revealed that a thick protein band appeared around 14.3KD in case of?B. cereus?treated with lead unlike the control samples.
机译：Metallothioneins（MTS）是一种富含半胱氨酸的金属结合蛋白，包括各种各样的生物，包括细菌，真菌以及所有真核植物和动物物种。 MTS粘合必要和非必需的重金属。通过简单的分光光度法评估MTS生产。该研究阐明了金属硫蛋白浓度在杆菌中的浓度β芽孢杆菌浓度。随着铅浓度的增加而增加，不同浓度的铅。来自βb.cereus的蛋白质样品？用不同浓度的铅处理，并在SDS-PAGE和2-D凝胶上电泳。对凝胶的研究显示，在βb的情况下，厚蛋白质带出现在约14.3kd左右。豆类？与对照样品不同铅处理。

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