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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Biological characterization of lead-resistant bacteria to explore role of bacterial metallothionein in lead resistance
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Biological characterization of lead-resistant bacteria to explore role of bacterial metallothionein in lead resistance

机译：铅耐药细菌的生物学特性，探讨细菌金属硫蛋白在铅耐药中的作用

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摘要

Lead-resistant bacterial isolates Salmonella choleraesuis strain 4A, Proteus penneri strain GM10, Bacillus subtillis strain GM02, Pseudomonas aeruginosa strain 4EA, Proteus penneri strain GM03 and Providentia rettgeri strain GM04 were isolated from soil contaminated with car battery waste from Goa, India. All the isolates except Pseudomonas aeruginosa strain 4EA showed presence of plasmids. Polymerase chain reaction amplification of 507 bp internal fragment of smtAB genes encoding bacterial metallothionein and intracellular bioaccumulation of 19 and 22 mg lead per gram dry weight in Salmonella choleraesuis strain 4A and Proteus penneri strain GM10 respectively revealed presence of metal-binding metallothionein (SmtA) being responsible for lead resistance encoded by genomic DNA. These lead-resistant and lead-bioaccumulating bacterial isolates can be employed for bioremediation of lead present in contaminated environmental sites.
机译：从印度果阿的汽车废电池污染的土壤中分离出耐铅细菌分离株霍乱沙门氏菌菌株4A，变形杆菌Pen10菌株GM10，枯草芽孢杆菌菌株GM02，铜绿假单胞菌4EA，变形杆菌Protenus penneri菌株GM03和Providentia rettgeri菌株GM04。除铜绿假单胞菌菌株4EA外的所有分离株均显示质粒的存在。编码细菌金属硫蛋白的smtAB基因507 bp内部片段的聚合酶链反应扩增以及霍乱沙门氏菌4A和变形杆菌Peneri菌株GM10中每克干重的细胞内生物积累19和22 mg铅每克干重，表明存在金属结合金属硫蛋白（SmtA）负责基因组DNA编码的铅抗性。这些抗铅和铅生物富集细菌分离株可用于污染环境中铅的生物修复。

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