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首页> 外文期刊>African Journal of Biotechnology >Identification of Lactobacillus pobuzihii from tungtap: A traditionally fermented fish food, and analysis of its bacteriocinogenic potential
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Identification of Lactobacillus pobuzihii from tungtap: A traditionally fermented fish food, and analysis of its bacteriocinogenic potential

机译：来自Tungtap的乳杆菌鉴定乳酸杆菌：传统发酵的鱼类食品，分析其细菌学潜力

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摘要

Lactic acid bacteria (LAB) were selectively isolated from tungtap, a traditionally fermented fish food product. Five such bacteria with bacteriocinogenic potential were characterized by polyphasic taxonomic approach. The phylogenetic analyses of 16S rRNA gene sequences showed their relatedness to?Lactobacillus pobuzihii(≥99.4% similarity), while biochemical and physiological characteristics revealed significant differences among the isolates. Bacteriocinogenic activity assay against selected bacterial strains:?Salmonella typhi?MTCC 733,?Bacillus cereus?MTCC 430,Klebsiella pneumoniae?MTCC 109,?Escherichia coli?MTCC 118 and?Bacillus licheniformis?MTCC 429 were performed with crude extracts using ethyl acetate as solvent, as well as by solvent and cell free supernatants. The growth inhibition zones were measured after incubation and compared with antibiotics like ampicillin, gentamicin, vancomycin, chloramphenicol and tetracycline. The culture extract of?L. pobuzihii?showed significant bacteriocinogenic activity against the tested strains.
机译：乳酸菌（实验室）选择性地从Tungtap中分离出一种传统发酵的鱼食品。通过多相分类方法表征五种具有噬菌体潜力潜力的这种细菌。 16S rRNA基因序列的系统发育分析表明它们的相关性与α乳酸杆菌（相似性≥99.4％），而生物化学和生理特性揭示了分离物之间的显着差异。对选定的细菌菌株的细菌学活性测定：？沙门氏菌Typhi？MTCC 733，λmtcc430，klebsiella pneumoniae？mtcc 109，Δscherichiacoli？mtcc 118和λ芽孢杆菌429与乙酸乙酯的粗提物一起进行。溶剂以及溶剂和无细胞上清液。孵育后测量生长抑制区，与氨苄青霉素，庆大霉素，万古霉素，氯霉素和四环素等抗生素相比。培养提取物的？l。 pobuzihii？显示出对测试菌株的显着的噬菌体活性。

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