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首页> 外文期刊>African Journal of Biotechnology >Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration
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Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration

机译：野生樱桃的增殖和生根：细胞素素和生长素类型的影响及其浓度

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Determination of the most optimal type and concentration of plant growth regulators as medium constituents is one of the most important aspects of successful micro propagation, among other?in vitro?factors. With the aim of optimization of?in vitromultiplication of wild cherry, the effect of the following cytokinins?was?studied: 6-benzyladenine (BAP), 2-isopentenyl adenine (2iP) and kinetin (Kin) at concentrations of 1, 2, 4 and 8 mg. l-1.?Stem segments of seedlings from juvenile and adult materials were disinfected and grown on a Quoirin and Lepoivre (1977) (QL) medium without growth regulators for 4 weeks. Each material responded differently to the tested cytokinins. The use of 6-benzyladenine resulted in the highest percentage of sprouting, the development of shoots and the ratios of multiplication for two materials of?Prunus avium?L. In the next experiment, seedlings from the juvenile and adult materials were grown on (MS2/5) medium in the presence of auxins indole-3-butyric acid (IBA), naphtaleneacetic acid (NAA), indole-3-acetic acid (IAA), when compared with concentrations of 0.5, 1, 2, and 4 mg. l-1. For the type of explants and its reactivity with the type and the concentration of auxin, significant differences among explants for root induction were observed. The adult material did not develop roots in any of the auxin and concentration used. In the case of the juvenile material, the IBA was distinguished from the other auxins tested and the highest induction of roots took place in 1 mg. l-1.?The most significant induction of cal characterizes, especially, the mediums containing the NAA followed by the IAA with concentrations of 2 and 4?mg. l-1, respectively,?which block the emergence of the roots partly and decreases the rate of rooting thereafter. The highest average number of roots and the highest?average length of roots?wereobtained with the IBA with 1?mg. l-1.
机译：作为培养基成分的植物生长调节剂最佳类型和浓度的测定是成功微繁殖的最重要方面之一，其中含有？体外呢？因素。旨在优化野生樱桃的vitroomultiplimation，以下细胞质素的效果？在浓度为1,2的浓度下研究：6-苄基腺嘌呤（BAP），2-异戊烯丁腺嘌呤（2IP）和运动蛋白（Kin）， 4和8毫克。 L-1。幼苗幼苗的幼苗和成人材料的幼苗在Quoirin和Lepoivre（1977）（QL）培养基上生长4周。每个材料与测试的细胞素响应不同。使用6-苄基腺嘌呤导致萌芽的最高百分比，芽的发育和两种材料的繁殖比率繁殖？在下一个实验中，来自幼年和成人材料的幼苗在菌吲哚-3-丁酸（IBA），萘酰乙酸（NAA），吲哚-3-乙酸（IAA）存在下（MS2 / 5）培养基），与0.5,1,2和4mg的浓度相比。 L-1。对于植物蛋白的类型和反应性，观察到根系诱导的外植体之间的显着差异。成人材料在使用的任何植物素和浓度中没有产生根部。在少年材料的情况下，IBA与所测试的其他性毒素区分开，并在1毫克中排出最高的根诱导。 L-1。当CAL的最显着诱导表征，特别是含有NAA的培养基，然后是IAA的浓度为2和4μg。 L-1分别，分别堵塞了根部的出现，并降低了此后的根源。最高的平均根数和最高的根部的根数？用1μmg用1μg含有IBA被染成的。 L-1。

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