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首页> 外文期刊>Acta Horticulturae >The Influence of Cytokinin and Auxin Types and Their Concentration on the Proliferation and Rooting of Viburnum treleasei Seedling Explants
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The Influence of Cytokinin and Auxin Types and Their Concentration on the Proliferation and Rooting of Viburnum treleasei Seedling Explants

机译：细胞分裂素和生长素的类型及其浓度对荚膜荚burn幼苗外植体增殖和生根的影响

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Recent demands on roadsides plantations and landscape restoration with native species along with species’ conservation, require huge amounts of individuals yet conserving their genetic diversity. This work aimed the establishment of Viburnum treleasei in vitro cultures from seedlings and the optimization of the in vitro shoots proliferation and rooting stages. With this purpose 500 embryos were isolated and inoculated on WPM (Woody Plant Medium). The seedlings nodal explants were then inoculated onWPM supplemented with 1.14, 4.56 and 22.81 muM of zeatin, 1.23, 4.92 and 24.6 muM of 2iP (N [2-isopentenyl] adenine) or 1.11, 4.44 and 22.2muM of BA (6-benzyladenine). After eight weeks, the nodal segments were subcultured in the same media formulations.Before root induction the plantlets were subcultured 8 weeks on WPM without growth regulators. Root induction was carried out by transferring plantlets (>1 cm) to a WPM supplemented with 0, 1.34, 5.37 and 10.74 fiM of NAA (a-naphthaleneacetic acid), 0,1.23, 4.92 and 9.84 muM of IBA (3-indolebutyric acid), or 0,1.43, 5.71 and 11.42 muM of lAA (3-indolacetic acid). The best proliferative responses of shoots in the two subcultures were obtained in the medium containing 22.81 muM zeatin. A significant increase of rooting percentages was only achieved on the media supplemented with NAA and after eight weeks the best rooting induction was achieved with 5.37 and 10.74 nM NAA.
机译：最近对路边人工林和原生物种的景观恢复以及物种保护的要求，要求大量个体同时保护其遗传多样性。这项工作的目的是建立从苗木中释放出的荚Vi荚and，并优化体外芽的增殖和生根阶段。为此目的，分离了500个胚，并接种在WPM（木本植物培养基）上。然后在补充了1.14、4.56和22.81μM玉米素，1.23、4.92和24.6μM2iP（N [2-异戊烯基]腺嘌呤）或1.11、4.44和22.2μMBA（6-苄基腺嘌呤）的WPM上接种幼苗节点外植体。八周后，将节段以相同的培养基配方进行亚培养。在诱导根之前，将小植株在无生长调节剂的WPM上亚培养8周。通过将幼苗（> 1 cm）转移至补充了0、1.34、5.37和10.74 fiM的NAA（α-萘乙酸），0.1.23、4.92和9.84μMIBA（3-吲哚丁酸）的WPM进行根诱导），或0.1.43、5.71和11.42μM的IAA（3-吲哚乙酸）。在包含22.81μM玉米素的培养基中获得了两种继代培养中芽的最佳增殖反应。仅在添加了NAA的培养基上，生根率才显着增加，八周后，用5.37和10.74 nM NAA达到了最佳的生根诱导。

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