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首页> 外文期刊>African Journal of Biotechnology >Studies of food thickeners in Nigeria for contamination by aflatoxigenic forms of Aspergillus and their detection by PCR
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Studies of food thickeners in Nigeria for contamination by aflatoxigenic forms of Aspergillus and their detection by PCR

机译：尼日利亚食物增稠剂的研究，用于曲霉形式的曲霉形式污染及其PCR检测

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This paper reports the contamination of ready-to-use food thickeners, collected from the South-East geo-political zone in Nigeria, by aflatoxigenic form ofAspergillus?species. A total of 150 samples from different open markets were observed for fungal contamination by using serial dilution-spread plate method. Although,?Aspergillus, Fusarium?and?Penicillium?were the most frequently isolated fungi,?Aspergillus?species were found to be the most prevalent in all the samples. Furthermore,?Aspergillus flavus?and?Aspergillus parasiticus?produced aflatoxin on yeast extract sucrose (YES) media incubated for 10 to 15 days at 27°C in a CO2incubator.?Aspergillus niger?showed no sign of any secondary metabolite on the media, set at similar conditions. Although, light microscopy was used to identify these fungi, based on colony morphology, PCR method was used to confirm genetic variation among the?Aspergillus?group, using ITS set of primers. Gel electrophoresis of PCR products confirmed the presence of?Aspergillus?species at an amplification range from 500 to 600 bp in all the samples tested. PCR was found to be a sensitive and a more reliable tool for detection and identification ofAspergillus?species in food thickeners as opposed to conventional light microscopy. This is a first kind of mycological survey on the contamination of ready-to-use food thickeners sold in Nigeria.
机译：本文报道了准备使用的食品增稠剂的污染，从在尼日利亚东南部的地缘政治区收集，由黄曲霉毒素的形式ofAspergillus？种。共有来自不同开放市场150个样品通过使用连续稀释扩展板方法观察真菌污染。虽然，？曲霉，镰刀菌？和？青？是最常见的孤立的真菌，？黑？被发现的物种是所有样品中最普遍的。此外，？黄曲霉α和β寄生曲霉？上产生酵母提取物蔗糖的黄曲霉毒素（YES）培养基中于27下温育10至15天℃的CO2incubator.?Aspergillus曲霉？显示在介质上没有任何次级代谢产物的迹象，定在类似的条件。虽然，光镜被用来确定这些真菌，根据菌落形态，使用PCR法来确认？曲霉？组中的遗传变异，使用其的引物组。 PCR产物的凝胶电泳证实的？曲霉存在？在500〜所有样品中为600bp的扩增范围的物种进行测试。 PCR被认为是一个敏感的和用于检测和鉴定ofAspergillus？在食品增稠剂物种，而不是常规的光学显微镜的更可靠的工具。这是第一种在尼日利亚销售的现成使用的食品增稠剂的污染真菌学调查。

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