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Antimicrobial potentials of Eclipta alba by disc diffusion method

机译:圆盘扩散法Eclipta Alba的抗微生物势

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This study was conducted to evaluate the antimicrobial potentials of methanol, petroleum ether, dichloromethane, ethyl acetate, butanol and water extracted samples from the aerial parts of?Eclipta alba?against nine microbial species. The antimicrobial (antibacterial and antifungal) susceptibility was screened by disc diffusion assay. The tested microbial species were?Bacillus cereus, Bacillus subtilis, Candida albicans, Erwinia carotovora, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi?and?Staphylococcus aureus. The extracts were applied in different concentrations of 1, 2 and 3 mg disc-1. Analysis of the data revealed that all the six extracts from?E. alba?showed different ranges of antimicrobial activities. Butanol fraction showed inhibitory activities against all the nine microbial species. Ethyl acetate fraction showed inhibitory effects against all the tested microbial species except?B. cereus?and?S. typhi. Petroleum ether, dichloromethane, methanol and water extracted samples had varying levels of inhibitions against some of these microorganisms. The most resistant microbial strain was?S. typhi?and was not controlled by petroleum ether, dichloromethane, methanol and water extracted samples. The most susceptible Gram positive bacterium was?B. subtilis?and was inhibited by all the six extracts from?E. alba?while the most resistant Gram positive bacterium was?B. cereus.?E. carotovora?was the most susceptible Gram negative bacterium while?S. typhi?andE. coli?were highly resistant among the Gram negative bacteria.
机译:进行该研究以评价甲醇,石油醚,二氯甲烷,乙酸乙酯,丁醇和水的抗微生物势来自来自葡萄节的空中部位的β-eClipta alba的水萃取样品吗?对九种微生物物种。通过盘扩散测定筛选抗微生物(抗菌和抗真菌和抗真菌)敏感性。经过测试的微生物物种?芽孢杆菌,枯草芽孢杆菌,念珠菌,念珠菌,伊尔维亚Carotovora,大肠杆菌,Klebsiella肺炎,假单胞菌铜绿素,沙门氏菌Typhi?和?葡萄球菌的葡萄球菌。萃取物以不同浓度的1,2和3mg盘-1施用。对数据的分析显示,所有六种提取物来自?e。 alba?显示出不同的抗菌活动范围。丁醇部分显示出对所有九种微生物物种的抑制作用。乙酸乙酯馏分对所有测试的微生物物种呈现抑制作用,除了Δb。豆火?和?s。 Typhi。石油醚,二氯甲烷,甲醇和水提取样品具有不同水平的对待这些微生物的抑制水平。最耐药的微生物菌株是Δs。 Typhi?并且没有由石油醚,二氯甲烷,甲醇和水提取的样品控制。最易感的革兰氏阳性细菌是Δb。枯草芽孢杆菌?被六种提取物抑制来自Δe。 alba?虽然最抵抗的革兰氏阳性细菌是?b。 Cereus. Carotovora?是最易感的革兰阴性细菌吗? typhi?ande。大肠杆菌?在革兰氏阴性细菌中具有高度抗性。

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