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METHOD FOR QUANTITATIVELY EVALUATING ANTIMICROBIAL ACTIVITY OF ANTIMICROBIAL SAMPLE USING DISC DIFFUSION METHOD AND SYSTEM FOR MEASURING ANTIMICROBIAL ACTIVITY USING THE SAME
METHOD FOR QUANTITATIVELY EVALUATING ANTIMICROBIAL ACTIVITY OF ANTIMICROBIAL SAMPLE USING DISC DIFFUSION METHOD AND SYSTEM FOR MEASURING ANTIMICROBIAL ACTIVITY USING THE SAME
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机译：碟片扩散法定量评价抗菌样品抗菌活性的方法及使用该方法测定抗菌活性的系统
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摘要
A method for quantitatively evaluating the antimicrobial activity of an antimicrobial sample using a disc diffusion method, and a measurement system using the method are provided. The method comprises: (a) measuring a maximum inhibition zone width (IZWmax) for a first sample, in which a colony is expected not to be observed, using a disc diffusion method, and measuring a colony forming unit (CFU0) value for the first sample using a colony counting method; (b) measuring a microbial growth inhibition zone width (IZWi) and a colony forming unit (CFUi) value for a second sample having any antimicrobial activity; (c) calculating α value and β value based on the results of steps (a) and (b) using equations (10) and (20); (d) measuring a microbial growth inhibition zone width (IZWx) for a measurement sample; and (e) calculating a quantitative antimicrobial activity value (η) using equation (30).
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机译：提供了一种使用盘扩散法定量评估抗菌样品的抗菌活性的方法，以及使用该方法的测量系统。该方法包括：（a）使用圆盘扩散法测量预期不会观察到菌落的第一样品的最大抑制区宽度（IZW max ），并测量菌落。采用菌落计数法形成第一个样品的单位（CFU 0 ）值; （b）测量具有任何抗菌活性的第二个样品的微生物生长抑制区宽度（IZW i ）和菌落形成单位（CFU i ）值; （c）使用等式（10）和（20）基于步骤（a）和（b）的结果计算α值和β值; （d）测量测量样品的微生物生长抑制区宽度（IZW x ）; （e）使用公式（30）计算定量的抗菌活性值（η）。
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