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首页> 外文期刊>African Journal of Biotechnology >Analysis of diversity genetic of Moroccan net blotch populations using amplified fragment length polymorphism (AFLP) markers
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Analysis of diversity genetic of Moroccan net blotch populations using amplified fragment length polymorphism (AFLP) markers

机译：使用扩增的片段长度多态性（AFLP）标记分析摩洛哥净污染群的多样性遗传

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摘要

Net blotch caused by?Pyrenophora teres f. teres?is the most harmful foliar disease in barley generating significant economic losses in Morocco. Populations of?P. teres f. teres?were collected from different regions of Morocco. Thirty five?(35)?P. teres f. teres?isolates, single conidial, were isolated and were subjected?to molecular study using amplified fragment length polymorphism (AFLP) technique. Out of?thefourteen primers combinations tested, four primers combinations were selected to disclose the polymorphism between the different?P. teres f. teres?isolates.?The molecular characterization of these isolates showed high degree of polymorphism reaching 95% and identifying 25 specific genotypes. The genetic variability of the different isolates of?P. teres f. teres?within and between Moroccan regions was highlighted, disclosing no linkage between the isolates and their geographical origins. This result might be due to informal material flow between regions.
机译：净污染由α斑纹斑纹F. TERES？是大麦中最有害的叶酸疾病在摩洛哥产生重大经济损失。群体？p。 teres f。特蕾莎？从摩洛哥的不同地区收集。三十五？（35）？p。 teres f。特蕾酸叶片？分离，单结合，进行分离并进行分子研究，使用扩增的片段长度多态性（AFLP）技术进行分子研究。出于α底漆的组合测试，选择四种引物组合以在不同的Δp之间公开多态性。 teres f。 TERES？分离物。这些分离株的分子表征显示出高度多态性达到95％并鉴定25个特定基因型。 βp不同分离株的遗传变异。 teres f。 TERES？突出显示摩洛哥地区之间，揭示了隔离物和地理起源之间的联系。该结果可能是由于地区之间的非正式材料流动。

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