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Baculovirus expression vector system: An efficient tool for the production of heterologous recombinant proteins

机译：Baculovirus表达载体系统：一种有效的制备异源重组蛋白的工具

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Today, the recombinant proteins become a major foundation of different industrial sectors. Products of these industries enhance the fields of medicine, pharmacology, agriculture, nutrition, petrochemicals, paper, textiles and many others. Since the mid 1980s, recombinant DNA technology has enabled the development of a wide diversity of vectors for expression of the desired recombinant protein in numerous prokaryotic and eukaryotic organisms. Baculovirus expression vector system is considered one of the most successful and widely acceptable means for?the?production of recombinant proteins in extremely large quantities. Proper posttranslational modifications of the expressed proteins in insect cells, the usual host of baculoviruses, get them soluble, correctly folded and biologically active products. Along with the convenient biological safety, the easy scale up production of recombinant proteins into industrial levels brings this system the first choice in most cases. In the current review, different aspects of the baculovirus biology, structure and morphology are briefly covered to establish the basic concepts important for understanding the mechanisms involved in utilization of baculoviruses as expression vectors. Few outlines on the baculovirus expression system are discussed in terms of potentials, limitations, strategies and perspectives.
机译：今天，重组蛋白成为不同工业部门的主要基础。这些行业的产品增强了医学，药理学，农业，营养，石化，纸，纺织品等田地。自20世纪80年代中期以来，重组DNA技术使得能够发展广泛的载体，以表达许多原核和真核生物中所需的重组蛋白。 Baculovirus表达载体系统被认为是最成功和广泛可接受的手段之一？在极大量中重组蛋白的产生。适当的昆虫细胞中表达蛋白质的正确性修饰，通常的杆状病毒宿主，使它们可溶，正确折叠和生物活性产品。随着方便的生物安全性，将重组蛋白的容易扩大到工业水平的生产使该系统在大多数情况下是第一个选择。在目前的审查中，简要覆盖了杆状病毒生物学，结构和形态的不同方面，以确定对理解利用杆状病毒作为表达载体的机制的基本概念。在潜在，限制，策略和观点方面，讨论了杆状病毒表达系统上很少有概述。

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