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Genetic diversity in Balkhi, Hashtnagri and Michni sheep populations using SSR markers

机译:使用SSR标记的Balkhi,Hashtnagri和Michni羊群的遗传多样性

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Morphological and genetic diversity among the three neighboring sheep breeds native to Central valley of Khyber Pukhtunkhwa (KP, Pakistan) was investigated. A total number of 138 non relative individuals of Balkhi (46), Hashtnagri (44) and Michni (48) was sampled for morphological as well as molecular characters using 31 ovine specific SSR markers.?Morphological observations and morphometric traits varied significantly among different sheep breeds. Balkhi having usually brown or white colour, with a tucked up fat tail was the larger breed. Hashtnagri is a medium sized breed; body covered with white wool, having long white tail, with a tail switch. The body colour of Michni sheep was usually brown or some times white. This breed is comparatively small in size with longer fat tail, hanging near (33.3%) or below (66.7%) hock. Total number of 119 alleles was identified with mean number of 3.8 alleles per locus, ranging from 2 to 8. Twelve unique alleles were identified in Michni population at different loci. Average gene diversity was higher in Michni (0.561). Inbreeding estimate (FIT) was significantly higher (27.1%) among three breeds and was highest between Balkhi and Hashtnagri (31%), similarlyhighest gene flow (Nm = 60.4) and lowest population differentiation (FST?= 4.3%) was estimated between these two breeds. Maximum genetic distance was observed between Balkhi and Michni; however, Balkhi was genetically closed to Hashtnagri population.?Balkhi and Michni were assigned at high accuracy to their respective population; however, the identity of Hashtnagri is obscure.
机译:调查了邻近牧羊地养殖的形态学和遗传多样性,其目的地对Khyber Pukhtunkhwa(KP,巴基斯坦)中央山谷(KP,巴基斯坦)进行了研究。对Balkhi(46),hashtnagri(44)和Michni(48)的总数的总数用于使用31个羊谱特异性SSR标记的形态学以及分子特征。在不同的绵羊之间具有显着变化的形态观察和形态学性状品种。 Balkhi经常有棕色或白色的颜色,塞满脂肪尾巴是较大的品种。 Hashtnagri是一种中型品种;身体覆盖着白色羊毛,长白色尾巴,带有尾部开关。 Michni绵羊的身体颜色通常是棕色或白色的一定时间。这种品种的尺寸相对较小,脂肪尾部较长,悬挂在近(33.3%)或以下(66.7%)赫克。 119个等位基因总数用平均每位基因座的数量为3.8个等位基因,范围为2至8个不同的等位基因在不同基因座的Michni群体中鉴定了12个独特的等位基因。 MICHNI(0.561)的平均基因多样性较高。三种品种的近亲繁殖估计(适合)显着高(27.1%),Balkhi和Hashtnagri(31%)之间的最高,类似地基因流(NM = 60.4)和最低种群分化(FST?= 4.3%)估计两种品种。 Balkhi和Michni之间观察到最大遗传距离;然而,Balkhi被遗传地关闭到Hashtnagri人口。?Balkhi和Michni以高精度分配给他们各自的人口;然而,Hashtnagri的身份是模糊的。

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