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首页> 外文期刊>African Journal of Biotechnology >Isolation of an ascorbate peroxidase in Brassica napus and analysis of its specific interaction with ATP6
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Isolation of an ascorbate peroxidase in Brassica napus and analysis of its specific interaction with ATP6

机译：芸苔内部插口中抗坏血酸过氧化物酶的分离及其与ATP6的特定相互作用分析

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In?Brassica napus, ATP6 is related to?Pol?cytoplasmic male sterility. To understand the mechanism of?Pol?CMS, proteins which interact with ATP6 were screened in a yeast two-hybrid system. A partial sequence of a putative?and?ascorbate peroxidase (Bn-APX)?was isolated from?Brassica napus. By use of rapid amplification of cDNA ends method, the full length of?Bn-APX?coding?sequence was cloned. The deduced amino acid sequence contained 438 amino acid residues with a conserved ascorbate peroxidase domain and shared 77% identity with that of?APX?fromArabidopsis thaliana. Further analyses revealed that the region of?Bn-APXinteracting with ATP6 was at its c-terminal. It was also observed that theexpressions of?ATP6?and?Bn-APX?were?strongly similar?in the?floral tissue?of CMS line and the restoring line. Furthermore, in restoring line, the expression of?Bn-APXis higher in the flower than that in other tissues.
机译：在？芸苔Napus，ATP6与β细胞质雄性不育有关。为了了解ΔCM的机制，在酵母双杂交系统中筛选与ATP6相互作用的蛋白质。一种推定的序列和α和α和α和α抗坏血酸过氧化物酶（BN-APX）？来自芸苔的乳房的β。通过使用CDNA结束方法的快速扩增，全长ΔBN-APX？克隆序列序列。所推导的氨基酸序列含有438个氨基酸残基，具有保守的抗坏血酸过氧化物酶结构域，并将77％的同一性与αFOFIABIDOPSIS的αFOFIANA。进一步分析显示，与ATP6的αbN-apx偶变区域在其C末端。还观察到了什么？ATP6？和？BN-APX？是什么相似？在？花组织？CMS线和恢复线。此外，在恢复线上，在花的α-apxis的表达比其他组织更高。

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