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首页> 外文期刊>African Journal of Biotechnology >Thylakoid-bound ascorbate peroxidase increases resistance to salt stress and drought in Brassica napus
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Thylakoid-bound ascorbate peroxidase increases resistance to salt stress and drought in Brassica napus

机译：类固醇结合的抗坏血酸过氧化物酶增加甘蓝型油菜对盐胁迫和干旱的抗性

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Reactive oxygen species (ROS) are cellular indicators of stress. In plants, they function as secondary messengers in response to environmental stress. Ascorbate peroxidase (APX) is an important enzyme directly involved in the scavenging of ROS. In this study, we aimed at identifying the function of the?Brassica napusthylakoid APX (tAPX). Germination efficiencies of seeds of?B. napus?plants over expressing tAPX were higher than those of the seeds of the control plants; this was true both on Murashige and Skoog medium with 300 mM mannitol and with 150 mM NaCl. Further experiments showed that 40-day-old seedlings of the control plants turned yellow, withered, and subsequently died, when treated with 150 mM NaCl for 12 days. In contrast, transgenic plants over expressing tAPX survived this treatment and had at least three green leaves at the end of the experiment. When 40-day-old seedlings were withheld water for 10 days, followed by a 2 day recovery, the control plants exhibited smaller leaves and shorter stems in comparison to tAPX-over expressing plants. In addition, compared with control plants, tAPX-overexpressing plants show reduced hydrogen peroxide accumulation and increased APX relative activity. Our results demonstrate that tAPX plays an important role in resistance to salt stress and drought in plants.
机译：活性氧（ROS）是压力的细胞指标。在植物中，它们充当响应环境压力的辅助信使。抗坏血酸过氧化物酶（APX）是直接参与清除ROS的重要酶。在这项研究中，我们旨在鉴定甘蓝型油菜核素APX（tAPX）的功能。 B.种子的发芽效率过表达tAPX的油菜植株高于对照植物的种子。在含有300 mM甘露醇和150 mM NaCl的Murashige和Skoog培养基上都是如此。进一步的实验表明，当用150 mM NaCl处理12天时，对照植物的40天大的幼苗发黄，枯萎并随后死亡。相反，过表达tAPX的转基因植物在该处理中幸存下来，并且在实验结束时至少具有三片绿叶。当将40天大的幼苗拒水10天，然后恢复2天时，与过表达tAPX的植物相比，对照植物的叶子较小，茎短。另外，与对照植物相比，过量表达tAPX的植物显示出减少的过氧化氢积累和增加的APX相对活性。我们的结果表明，tAPX在植物抗盐胁迫和干旱中起重要作用。

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