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首页> 外文期刊>African Journal of Biotechnology >The isolation and amplification of full length cDNA of oleosins from oil palm (Elaeis guineensis Jacq.)
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The isolation and amplification of full length cDNA of oleosins from oil palm (Elaeis guineensis Jacq.)

机译：油棕（Elaeis Guineensis Jacq）的疏油素全长cDNA的分离和扩增。

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This study focused on the isolation and amplification of the cDNA clone of oleosin from oil palm. The oil palm cDNA library constructed from the kernel tissues 10 weeks after anthesis (WAA) produced a clone of pO1A containing partial 563 bp sequence of oleosin. The polymerase chain reaction for the rapid amplification of cDNA ends (RACE-PCR) was performed to obtain full length cDNA of oleosin from the RNA transcripts using forward gene-specific primers that is, primer Oleo 3. The product was then cloned into pCR 4 TOPO vector and subjected to sequencing using M13 forward and reverse primers. The end-to-end PCR method was performed to amplify the complete cDNA sequence of oleosin and it produce a 750 bp PCR product. The BLAST results showed that the cDNA from oil palm kernel isolated exhibited high similarities with low molecular weight isoforms of oleosin cDNA from crops like rice, maize and barley.?Keywords:?Oleosin, oil palm, cDNA, RACE-PCR, gene-specific primers.
机译：本研究专注于从油棕中分离和扩增油糖蛋白cDNA克隆。在开花（WAA）后10周由籽粒组织构成的油棕cDNA文库制备了含有含有部分563bp序列的油蛋白的PO1a的克隆。用于快速扩增cDNA末端的聚合酶链反应（RAP-PCR）以使用前进基因特异性引物从RNA转录物中获得来自RNA转录物的全长cDNA，即引物Oleo 3.然后将产物克隆到PCR 4中Topo载体并使用M13向前和反向引物进行测序。进行端到端PCR方法以扩增油蛋白的完整cDNA序列，并产生750bp PCR产物。爆炸结果表明，孤立的油棕榈核的cDNA与水稻，玉米和大麦等作物的油蛋白cDNA的低分子量同种型表达了高相似性.?:::::Oleosin，油棕，cDNA，种族PCR，基因特异性引物。

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