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The incidence and antibiotics susceptibility of Escherichia coli O157:H7 from beef in Ibadan Municipal, Nigeria

机译:大肠杆菌O157的发病率和抗生素易感性:H7来自尼日利亚尼日利亚市牛肉牛肉

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The incidence of?Escherichia coli?0157: H7 was assessed in meat samples from slaughtered cattle in Ibadan metropolis by culturing on sorbitol MacConkey agar and confirmed using serological agglutination kits. The?isolates were tested for susceptibility to seven commonly used antimicrobial agents. Out of the total of the 116 non-sorbitol fermenting (NSF)?E. coli?isolated from the samples, 71 (comprising of 18.4, 2.0, 3.2 and 4.8% from Bodija abattoir, Bashorun, Apata and Iwo Road slaughter slabs, respectively) were confirmed as?E. coli?O157:H7 serotype. Antibiotics susceptibility profile showed that all the isolates were resistant to one or multiple antibiotics, resulting in eight different resistance patterns. Tetracycline resistant isolates were the highest with 91.4% incidence. The results are of public health significance confirming cattle as major reservoir of EHEC and antimicrobial-resistant organisms. The high level of carcass contamination with microorganisms may be due to unhygienic slaughtering and meat processing engaged in these abattoir and slabs. Indiscriminate and misuse of antimicrobials on livestock in Nigeria could also be responsible for the multiple resistance pattern of the organism. Application of food hygiene practices such as HACCP is recommended for high quality farm to fork wholesome and safe meat for public consumption in Nigeria.
机译:通过培养山梨糖醇Macconkey琼脂,在山梨糖醇Macconkey琼脂中培养并确认使用血清学凝集试剂盒进行证实,在肉类样本中评估了屠宰牛的肉样品中的肉样本中的发病率。测试分离物对七种常用抗菌剂的敏感性。出于116个非山梨糖醇发酵(NSF)的总和出来?e。 Coli?分别从样品中分离,71(分别包含来自Bodija Abattoir,Bashorun,Apata和IWO道路屠宰板的Bashorun,Apata和IWO路屠宰板)。 COLI?O157:H7血清型。抗生素敏感性谱表明,所有分离株均对一种或多种抗生素抵抗力,导致八种不同的电阻模式。四环素抗性分离物是最高的91.4%的发病率。结果是公共健康意义,证实牛作为EHEC和抗菌生物的主要储层。具有微生物的高水平胴体污染可能是由于非否尼屠宰和肉类加工,从事这些屠宰场和板坯。尼日利亚牲畜上的抗菌药物滥用和滥用抗菌药物也可能对生物体的多种抗性模式负责。为HACCP等食品卫生实践的应用,为高质量的农场推荐用于叉子有益健康,尼日利亚的公共消费。

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