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首页> 外文期刊>African Journal of Biotechnology >Morphological and proteomic analyses of Zea mays in response to water stress
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Morphological and proteomic analyses of Zea mays in response to water stress

机译：Zea的形态学和蛋白质组学分析响应水分胁迫

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Water stress affects plant growth and development, leading to agricultural crop losses in maize cultivation. It also threatens food security in economical crops such as maize, one of the major crops produced worldwide. Transcriptomic studies associated with morphological assessments have been widely conducted on the mechanisms of crop development and stress response; however, data on maize is still very much limited. Hence herein, we used both the morphological and proteomic analyses to investigate and establish physical features and proteins associated with maize in response to osmotic stress. In addition, proteomic analysis (1DE and 2DE techniques) was used to separate and enumerate water stress responsive proteins. Morphologically, a decrease in the overall growth of the maize plant as a result of water stress was observed, whereby features such as leaf colour and size, shoot height and stem diameter were negatively affected. Through proteomics analyses, a total of nine expressed proteins were revealed in response to water stress. Overall, this work, has successfully profiled the water stress responsive proteins and specifically indicating the efficiency of proteomic tools in the detection and analysis of qualitative proteins from maize.
机译：水分压力影响植物生长和发展，导致玉米种植中的农业作物损失。它还威胁到玉米经济作物中的粮食安全，这是全球生产的主要作物之一。与形态学评估相关的转录组研究已被广泛对作物发展和应力反应的机制进行;但是，玉米数据仍然非常有限。因此，在本文中，我们使用形态学和蛋白质组学分析来研究和建立与玉米相关的物理特征和蛋白质，响应于渗透胁迫。此外，蛋白质组学分析（1DE和2DE技术）用于分离和枚举水应激响应蛋白。在形态上，观察到由于水分应激而导致玉米植物的总体生长的降低，其中叶子颜色和尺寸等特征受到负面影响。通过蛋白质组学分析，响应于水胁迫显示总共九种表达蛋白质。总体而言，这项工作成功地成功地分析了水分应激响应蛋白，特别是表明蛋白质组学工具在玉米的定性蛋白质检测和分析中的效率。

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