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首页> 外文期刊>African Journal of Biotechnology >Comparative study of single nucleotide polymorphisms (SNPs) of a candidate growth gene (IGF-I) in Oreochromis niloticus and Sarotherodon melanotheron
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Comparative study of single nucleotide polymorphisms (SNPs) of a candidate growth gene (IGF-I) in Oreochromis niloticus and Sarotherodon melanotheron

机译：奥海霉菌含NiLoticus患者候选生长基因（IGF-I）的单核苷酸多态性（SNP）的比较研究

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摘要

In teleost fishes, the regulation of growth performance by the GH and IGF system also seems to be highly conserved. Adult Oreochromis niloticus (Nile tilapia) could reach up to 60 cm maximum length while Sarotherodon melanotheron (black chin Tilapia) has a maximum length of 28 cm. This study describes the analysis of Single Nucleotide Polymorphism in isolated, amplified, and sequenced DNA from two common Tilapia species (O. niloticus and S. melanotheron) with the aim of identifying genetic variation and single nucleotide polymorphisms (SNPs) in one of the main genes, Insulin like growth Factor-1 (IGF-I), related to growth in the Tilapia species. The extracted DNA from the clipped caudal fins of the Tilapia species samples using Sambrook and Russell’s modified chlolorophenol/isoamyl alcohol DNA extraction method were further amplified in a thermal cycler with designed IGF-I forward and reverse primers of 447 bp which were subsequently sequenced with an automated analyzer. The PCR product was separated on 1.5% ethidium stained agarose gel electrophoresis and the bands revealed on the gel were all of the same length (447 bp). The Sequence alignment revealed a total of five single nucleotide polymorphisms which were detected in the forward reaction at the positions 181, 199, 328, 362 and 369 of the sequences and in the reverse reaction at positions 18, 20, 54, 183 and 201. A total of 138 amino acid sequence was translated from the DNA sequence with variations sequence at positions 1, 3, 4, 61, 67, 121 and 123. These results showed variations among these two fish species which could explain differential growth performance between them.
机译：在Textost鱼类中，GH和IGF系统的增长性能调整似乎也受到高度保守。成人Oreochromis niloticus（尼罗替尼亚岛）最长可达60厘米的长度，而萨拉莫顿·梅拉洛仑（黑冠罗非鱼）的最大长度为28厘米。本研究描述了分离，扩增和测序的单核苷酸多态性的分析，来自两种常见的罗非鱼物种（O. niloticus和S. Melanothoth）的目的是鉴定遗传变异和单个核苷酸多态性（SNP）其中一个主要基因，胰岛素如生长因子-1（IGF-1），与罗非鱼种类的生长有关。来自罗布亚洲物种样品的夹层尾部的提取的DNA使用Sambrook和Russell的改性碳氢苯酚/异戊醇DNA提取方法在热循环仪中进一步扩增，其设计的IGF-I正向和反向引物为447bp，随后测序自动分析仪。在1.5％乙状符上分离PCR产物染色琼脂糖凝胶电泳，凝胶上显示的带状均为相同的长度（447bp）。序列对准显示了总共五种单核苷酸多态性，其在序列的位置181,199,328,362和369处的前向反应中检测，并且在位置18,20,54,183和201的反应中。通过在第1,3,4,61,67,121和123的位置，总共138个氨基酸序列从DNA序列转换，具有变化序列。这些结果表明这两种鱼类中的变化可以解释它们之间的差异增长性能。

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