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首页> 外文期刊>African Journal of Biotechnology >First report of mortality in Phoenix canariensis associated with subgroup 16SrIV‐D phytoplasmas in Coahuila, Mexico
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First report of mortality in Phoenix canariensis associated with subgroup 16SrIV‐D phytoplasmas in Coahuila, Mexico

机译：第一份与Coahuila，墨西哥Coahuila亚组16sriv-d植物相关的Phoenix Canariensis的死亡率

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In 2015, a disease of unknown origin appeared in Torreon, Coahuila, in the Northeast of Mexico, causing great mortality in Phoenix palms, especially in Phoenix canariensis. Until early 2019, around 1300 palms died from this disease. The aim of this study was to determine its etiology. The symptoms registered in affected palms were similar to those described for Texas Phoenix Palm Decline (TPPD). Phytoplasmas were detected in samples from nine P. canariensis individuals using a TaqMan/real-time PCR assay specific for group 16SrIV detection. DNA of positive samples was amplified by nested PCR using primer pair P1/P7 followed by LY16Sf/LY16-23Sr and R16F2n/R16R2. In silico analysis of the sequences obtained revealed the presence of phytoplasmas associated with TPPD, belonging to subgroup 16SrIV-D. This is the first report of a disease associated with subgroup 16SrIV-D phytoplasmas in the Northeast of Mexico, further extending the known geographical range of this pathogen.
机译：2015年，在墨西哥东北部的Coahuila的Torreon出现了一种未知的起源疾病，在凤凰棕榈树上造成了巨大的死亡率，特别是在凤凰Canariensis中。直到2019年初，大约1300只棕榈树死于这种疾病。本研究的目的是确定其病因。在受影响的棕榈树中注册的症状类似于德克萨斯州凤凰棕榈衰退（TPPD）所描述的症状。使用针对组16SRIV检测的Taqman /实时PCR测定法在来自九种P. Canariensis个体的样品中检测到植物。使用引物对P1 / P7通过巢式PCR扩增阳性样品的DNA，其次是Ly16SF / Ly16-23SR和R16F2N / R16R2。在获得的序列中的硅分析中，揭示了与TPPD相关的植物存在，属于亚组16SRIV-D。这是墨西哥东北部与亚组16SRIV-D植物相关的疾病的第一个报告，进一步延长了该病原体的已知地理范围。

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