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首页> 外文期刊>American Journal of Analytical Chemistry >Derivative Spectrophotometric and Isocratic High Performance Liquid Chromatographic Methods for Simultaneous Determination of Repaglinide and Metformin Hydrochloride in Pharmaceutical Preparations
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Derivative Spectrophotometric and Isocratic High Performance Liquid Chromatographic Methods for Simultaneous Determination of Repaglinide and Metformin Hydrochloride in Pharmaceutical Preparations

机译：衍生分光光度法和等异构高性能液相色谱法，用于同时测定药物制剂中盐酸盐和盐酸二甲双胍

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In this study, a derivative spectrophotometric method and one HPLC method were developed and validated for analysis of anti-diabetic drugs, repaglinide (RPG) and metformine hydrochloride (MTF) in tablets. The spectrophotometric methods were based on zero-crossing first-derivative and fourth-derivative spectrophotometric method for simultaneous analysis of RPG (308 nm) and MTF (267 nm), respectively. Linear relationship between the absorbance at λmax and the drug concentration was found to be in the ranges of 5.0 - 50.0 μg style="white-space:nowrap;">·mL-1 for both RPG and MTF. The quantification limits for RPG and MTF were found to be 0.568 and 1.156 μg style="white-space:nowrap;">·mL-1, respectively. The detection limits were 0.170 and 0.347 μg style="white-space:nowrap;">·mL-1 for RPG and MTF, respectively. The second method is a rapid stability-indicating isocratic HPLC method developed for the determination of RPG and MTF. A linear response was observed within the concentration range of 5.0 - 50.0 μg style="white-space:nowrap;">·mL-1 for both RPG and MTF. The quantification limits for RPG and MTF were found to be 1.821 and 1.653 μg style="white-space:nowrap;">·mL-1, respectively. The detection limits were 0.601 and 0.545 μg style="white-space:nowrap;">·mL-1 for RPG and MTF, respectively. The proposed methods were successfully applied to the tablet analysis with good accuracy and precision.
机译：在该研究中，开发并验证了一种衍生分光光度法和一种HPLC方法，用于分析片剂中的抗糖尿病药物，常规盐酸盐（RPG）和盐酸二甲盐（MTF）。分光光度法是基于零交叉的第一衍生物和第四衍生物分光光度法，分别同时分析RPG（308nm）和MTF（267nm）。发现在λ max 和药物浓度之间的线性关系是5.0-50.0μg<跨度样式=“白色空间：nowrap;”的范围。 >· ml -1 用于rpg和mtf。 RPG和MTF的定量限值被发现为0.568和1.156μg<跨度样式=“白色空间：Nowrap;”>· ml -1 -1 。检测限为0.170和0.347μg<跨度样式=“白色空间：nowrap;”>·· ml -1 / sup>用于RPG和MTF。第二种方法是一种快速稳定性指示，用于测定RPG和MTF的均匀性HPLC方法。在5.0-50.0μg<跨度=“空白空间：nowrap;”>· ml -1 -1 / sup>的浓度范围内观察到线性响应，用于rpg和mtf。 RPG和MTF的定量限制被发现为1.821和1.653μg<跨度样式=“白色空间：nowrap;”>· ml -1 。检测限为0.601和0.545μg<跨度样式=“白色空间：nowrap;”>· ml -1 -1 用于RPG和MTF。提出的方法以良好的准确性和精度成功地应用于片剂分析。

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