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Purification of Nanoparticles by Liquid Chromatography for Biomedical and Engineering Applications

机译:通过液相色谱法纯化纳米颗粒,用于生物医学和工程应用

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Nanoparticles are extensively used for various applications in science, engineering and medicine. Synthesis of nanoparticles with high purity is essential to utilize the same in different fields of science and technology. In the present study, liquid chromatography is utilized to purify the nanoparticles. Predominantly, gold nanoparticles were synthesized from gold auric cholide and preserved in phosphate or citrate buffer. A method to purify gold nanoparticles is essential because of the possible interference from gold auric chloride and other impurities in buffer. Herein, a method has been developed using high performance liquid chromatography to purify gold nanoparticles with 100 nm in size from gold auric chloride and residues. UV-Vis spectroscopy was also done to ascertain the purity of the nanoparticles.
机译:纳米粒子广泛用于科学,工程和医学中的各种应用。具有高纯度的纳米颗粒的合成对于在不同的科学和技术领域中利用相同是必不可少的。在本研究中,利用液相色谱纯化纳米颗粒。主要是,金纳米颗粒由金抗硫化物合成,并保存在磷酸盐或柠檬酸盐缓冲液中。纯化金纳米颗粒的方法是必不可少的,因为可能的缓冲液中的金抗氯化物和其他杂质的干扰。在此,使用高效液相色谱法开发了一种方法,以从金抗氯化物和残基的尺寸纯化金纳米颗粒。还进行了UV-Vis光谱以确定纳米颗粒的纯度。

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