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首页> 外文期刊>American Journal of Analytical Chemistry >Validation and Comparison of Two Calibration Methods for the Measurement of Stable Iodine in the Urinary Matrix by ICP-MS: Standard Addition vs. External Calibration
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Validation and Comparison of Two Calibration Methods for the Measurement of Stable Iodine in the Urinary Matrix by ICP-MS: Standard Addition vs. External Calibration

机译：ICP-MS测量尿基稳定碘稳定碘校准方法的验证及比较：标准加法与外部校准

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Background: In the context of a nuclear reactor accident, thyroid is the main target organ of radioactive iodines. To avoid as much as possible thyroid disorders or even cancer development, it is recommended to administer a single dose of potassium iodide to people at risk of exposure. Nevertheless, the Fukushima Dai-ichi disaster has pointed out many questions about the conditions of stable iodine prophylaxis implementation highlighting the need for reflection further revision of the actual “iodine doctrine”. Therefore, providing useful data is required notably through the implementation of animal experiments to strengthen current knowledge and to edit new recommendations. Methods: Urinary iodine constitutes a very good indicator to investigate the function of thyroid, its interpretation demands reliable analyses. Prior to perform animal experiments, two calibration methods were designed by our lab and compared together (standard addition and external calibration) to assess the urinary concentration of stable iodine in urine by ICP-MS. They were validated based on several key parameters especially linearity, accuracy and limits of detection (LOD) and quantification (LOQ). Results: The results were nicely satisfying. Indeed, both calibration methods have indicated very good coefficients of correlations, accuracies with low expanded relative uncertainties were obtained. The estimated LOD in the sample for standard addition method and external calibration were fully acceptable, 0.39 μg·L-1 and 0.35 μg·L-1, respectively. All performance criteria have been thus fulfilled successfully. The established methods were proven to be accurate, robust and sensitive. Once validated, both calibration methods were applied to rat urine samples and the results of z-score and Wilcoxon W test concluded that there were no statistically significant differences between both methods.
机译：背景：在核反应堆事故的背景下，甲状腺是放射性碘的主要目标器官。为避免尽可能多的甲状腺障碍甚至癌症发展，建议使用暴露风险的人们向人们施用单剂量的碘化物。尽管如此，福岛傣族灾难已经指出了关于稳定碘预防稳定的条件的疑问，突出了对实际“碘学说”的反思进一步修订的需要。因此，显着通过实施动物实验来提供有用的数据来加强当前知识并编辑新的建议。方法：尿碘构成一个非常好的指标，探讨甲状腺的功能，其解释需要可靠的分析。在执行动物实验之前，我们的实验室设计了两种校准方法，并将其与其一起进行（标准添加和外部校准），以评估ICP-MS尿液中尿液中稳定碘的尿液浓度。基于几种关键参数验证，特别是线性度，检测精度和限制（LOD）和量化（LOQ）。结果：结果令人满意。实际上，两种校准方法都表明了非常好的相关系数，获得了低扩增的相对不确定性的精度。标准添加方法和外部校准样品中的估计床位是完全可接受的，0.39μg·L-1和0.35μg·L-1。因此，所有性能标准都已成功实现。已证明建立的方法是准确的，稳健和敏感的。一旦经过验证，将两种校准方法施用于大鼠尿液样品，并且Z-得分和Wilcoxon W测试的结果得出结论，两种方法之间没有统计学上显着的差异。

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