• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Analytical Chemistry >Is Titration as Accurate as HPLC for Determination of Vitamin C in Supplements? &br/&—Titration versus HPLC for Vitamin C Analysis
【24h】

Is Titration as Accurate as HPLC for Determination of Vitamin C in Supplements? &br/&—Titration versus HPLC for Vitamin C Analysis

机译：滴定是作为HPLC的准确性，用于测定补充剂中的维生素C？ ＆ br /＆ - 用于维生素c分析的itusus vplc

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Herein, the iodometric titration and HPLC-RP methods were compared for the determination of vitamin C in vitamin and mineral supplements. The methods were validated in terms of linearity, limits of detection (LOD), limits of quantification (LOQ), precision, and recovery by using vitamin standards and a reference material SRM 3280 (multivitamin/multimineral tablets) obtained from the National Institute of Standards and Technology (NIST). A total of 22 samples of vitamin supplements, randomly acquired in local markets of Sao Paulo (Brazil) were evaluated for content of vitamin C by these two methodologies. The precision expressed as RSD was lower than 5% for both methods. LOD was 3.6 μg/mL for HPLC and 1.0 mg for titration, while LOQ were 12.0 μg/mL and 3.0 mg for HPLC and titration, respectively. Percent recoveries (%) of spiked samples ranged from 98.7 to 100.5 for HPLC and from 98 to 104 for titration. The contents of vitamin C in SRM 3280 (Certified value = 42.2 ± 3.7 mg/g) were 40.2 and 42.1 mg/g when determined by HPLC and titration respectively. Statistically, there was no difference in the analysed vitamin C content for half of the samples, irrespective of the method applied. HPLC was more sensitive, but the titrimetric method was faster and consumed less reagent. Although both methods were accurate in determining the vitamin C content in SRM 3280, the matrix constituents of some vitamin supplements may have interfered with the analysis.
机译：在此，比较碘滴定和HPLC-RP方法，用于测定维生素和矿物质补充剂中的维生素C.通过使用维生素标准的方法，检测限，定量限制（LOD），量化限制（LOQ），精度和恢复，从国家标准研究所获得的参考材料（多种维生素/多内一体片），验证了这些方法。和技术（NIST）。通过这两种方法评估了在Sao Paulo（巴西）的当地市场中随机获得的22种维生素补充剂样品，对维生素C的含量评估了维生素C的含量。两种方法表示为RSD的精度低于5％。对于HPLC为3.6μg/ mL，滴定为1.0mg，滴定为1.0mg，而LOQ分别为12.0μg/ mL和3.0mg，用于HPLC和滴定。掺入样品的百分比回收率（％）为HPLC的98.7-100.5和滴定的98-104。当通过HPLC和滴定法测定，SRM 3280（认证值= 42.2±3.7mg / g）中维生素C的含量为40.2和42.1mg / g。统计上，随着所施加的方法，分析的维生素C含量没有分析的维生素C含量没有差异。 HPLC更敏感，但滴定法较快，试用较少。尽管两种方法在确定SRM 3280中的维生素C含量方面是准确的，但是一些维生素补充剂的基质成分可能受到干扰的分析。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号