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首页> 外文期刊>American Journal of Analytical Chemistry >Determination of Vitamin B12 Using Differential Pulse Polarography
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Determination of Vitamin B12 Using Differential Pulse Polarography

机译：使用差分脉冲极谱法测定维生素B12

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Vitamin B12 is a type of vitamin also known by the name cobalamin. B12 is involved in many metabolism activities, including DNA synthesis, nervous system, red blood formation and immune system. Therefore, we chose the Differential Pulse Polarography (DPP) method is that has a high sensitivity for the determination of vitamin B12. This determination was possible with cobalt present in vitamin B12 structure. Since Co(III) is formed from the oxidation of the vitamin, its polarographic behavior had to be determined in various electrolytes such as acetate, borate, phosphate and ammonia. The polarograms of Co(III) were taken in these electrolytes in which 1.0 M NH3/ (pH = 9.8) and 1.0 M AcOH/AcO~(-) (pH = 4.0) were found as the most suitable electrolytes. This method was successfully applied vitamin of B12 determination in a 1 mL ampoule with high precision. The LOD was found as 3.7 × 10~(-7) for instead of (S/N = 3). Besides Co(III), interference effects of Zn(II), Ni(II), Cr(III), Fe(III), Cu(II), Cd(II) and Se(IV) were also studied. It was found that only Zn(II) peak had an overlap Co(III) peak in ammonium buffer. This problem could be solved by working in 1.0 M AcOH/AcO~(-) (pH = 4.0) buffer. B12, which is 1000 μg in 1 mL vitamin ampoule, was found for 4 measurements as 999 ± 15 μg as a result of 95% confidence interval.
机译：维生素B12是钴胺名称也已知的维生素。 B12涉及许多新陈代谢活动，包括DNA合成，神经系统，红血浆形成和免疫系统。因此，我们选择差分脉冲极谱（DPP）方法是对维生素B12的测定具有高灵敏度的方法。在维生素B12结构中存在的钴可以使用该测定。由于CO（III）由维生素的氧化形成，因此必须在各种电解质如乙酸盐，硼酸盐，磷酸盐和氨中确定其极谱行为。在这些电解质中截取CO（III）的极化图，其中1.0M NH 3 /（pH = 9.8）和1.0M acoh / aco〜（ - ）（pH = 4.0）作为最合适的电解质。该方法成功地应用了B12的维生素，高精度为1ml安瓿中的1ml测定。发现LOD为3.7×10〜（-7）代替（s / n = 3）。此外，还研究了CO（III），还研究了Zn（II），Ni（II），Cr（III），Fe（III），Cu（II），CD（II）和SE（IV）的干扰作用。发现只有Zn（II）峰在铵缓冲液中具有重叠CO（III）峰。该问题可以通过在1.0 m acoh / aco〜（ - ）（pH = 4.0）缓冲液中工作来解决。在1mL维生素安瓿中为1000μg的B12，发现4次测量为999±15μg，由于95％的置信区间。

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