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Application of Differential Pulse Polarography for Detection of Riboflavin from Pharmaceutical Samples and Vegetables

机译:差分脉冲极谱法在药物样品和蔬菜中检测核黄素的应用

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Riboflavin (vitamin B2) plays an important role in living processes as a precursor of coenzymes. The core structure of riboflavin with isoalloxazine ring participates in enzyme-catalyzed electron transfer processes of many important metabolites. Several methodologies have been developed for the determination of riboflavin, but those methods have disadvantages like long analysis time, consumption of a lot of reagents, and expensiveness. Hence, to overcome these problems, the DPP technique is proposed in this study. The DPP method is used for the quantitative analysis of riboflavin. The experimental conditions are optimized to obtain the best characterized peak in terms of peak height with analytical validation of the method. The proposed methods are applied for the analysis of riboflavin in pharmaceutical samples and vegetables. The riboflavin is found to adsorb and undergo irreversible reduction reaction at the working mercury electrode.
机译:Riboflavin(维生素B2)在生活过程中发挥着重要作用作为辅酶的前兆。核黄素与异唑嗪环的核心结构参与了许多重要代谢物的酶催化电子转移过程。已经开发了几种方法来测定核黄素,但这些方法具有类似于长分析时间,消耗大量试剂的缺点,以及大量的缺点。因此,为了克服这些问题,在本研究中提出了DPP技术。 DPP方法用于核黄素的定量分析。实验条件经过优化,以便在峰值高度方面获得最佳特征的峰值,具有方法的分析验证。所提出的方法用于分析药物样品和蔬菜中的核黄素。发现核黄素在工作汞电极处吸附并经历不可逆还原反应。

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