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首页> 外文期刊>Australian Journal of Crop Science >Morphophysiological analysis and expression of proline genes in rice (Oryza sativa L. cv. BRS AG) subjected to in vitro salt stress
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Morphophysiological analysis and expression of proline genes in rice (Oryza sativa L. cv. BRS AG) subjected to in vitro salt stress

机译：在体外盐胁迫下水稻中脯氨酸基因的（Oryza Sativa L.Cv.Brs Ag）的语无态生物学分析及表达

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About 20% of the cultivated land and almost half of the irrigated lands of the planet are affected by the salinization of the soil and the water. Rice cultivar BRS AG is the first grown for other purposes than human consumption and can be used in the production of ethanol and animal feed. The objective of this study was to evaluate the germination, morphological characteristics, proline accumulation, and the expression of the genes involved in the metabolism of proline in rice plants, cv. BRS AG, cultured in vitro under salt stress. The plants were cultivated in a culture MS medium with six different NaCl concentrations (0, 32, 68, 102, 136, 170 mM). The germination was evaluated at days 7 and 14, and morphological characteristics were analyzed only at day 21. For proline quantification and expression of the genes required for its metabolism (P5CS1, P5CS2, P5CR, OAT, P5CDH and PDH), two concentrations (136 mM and 0 mM) of NaCl were used, and the samples were collected at days 5, 10, 15, and 20. The results indicate that the growth of rice cv. BRS is completely impaired at 170 mM NaCl, and at 136 mM NaCl. Regarding proline content, in stressed plants, it was higher (1.24 mM g -1 MF) than in the control plants, and was directly related to a high expression observed for the P5CS2 (QR=5.50) and OAT (QR=5.44) genes at 20 days of stress, which explains the high proline concentration at this period. According to the results, rice cultivar BRS AG, is considered tolerant to salt stress.
机译：大约20％的耕地和地球的灌溉土地的几乎一半受到土壤和水的盐渍化的影响。水稻品种BRS AG是第一次用于其他目的而不是人类消费，可用于生产乙醇和动物饲料。本研究的目的是评估萌发，形态学特征，脯氨酸积累和参与水稻植物中脯氨酸代谢的基因的表达，CV。 BRS AG，在盐胁迫下在体外培养。在培养MS培养基中培养植物，六种不同的NaCl浓度（0,32,68,102,136,170mM）。在第7天和第14天评估萌发，并且仅在第21天分析形态特征。对于其新陈代谢所需的基因的脯氨酸定量和表达（P5CS1，P5CS2，P5CR，OAT，P5CDH和PDH），两种浓度（136使用Mm和0 mm），并在第5,10,15和20天收集样品。结果表明水稻CV的生长。 BRS在170mM NaCl和136mM NaCl下完全损害。关于脯氨酸含量，在压力植物中，它比对照植物更高（1.24mmg -1mF），并且与P5CS2（QR = 5.50）和燕麦（QR = 5.44）基因直接相关的高表达有关在20天的压力下，在此期间解释了高脯氨酸浓度。根据结果​​，水稻品种BRS AG被认为耐盐胁迫。

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