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首页> 外文期刊>Australian Journal of Crop Science >Characterization of maintainer and restorer lines for wild abortive cytoplasmic male sterility in indica rice (Oryza sativa L.) using pollen fertility and microsatellite (SSR) markers
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Characterization of maintainer and restorer lines for wild abortive cytoplasmic male sterility in indica rice (Oryza sativa L.) using pollen fertility and microsatellite (SSR) markers

机译：用花粉生育率和微卫星（SSR）标记，籼稻（Oryza Sativa L.）野生流动细胞质雄性不育的维护器和恢复系的特征

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摘要

A narrow genetic base and insufficient numbers of parental lines are major constraints in the development of location-specific hybrid rice varieties. We have studied 4 wild abortive (WA) cytoplasmic male sterile (CMS) lines and 45 pollen parents of diverse origins in order to identify potential restorers and maintainers using test crosses. Pollen viability tests from 85 successful crosses were able to differentiate parental lines into restorers, partial restorers, partial maintainers, and maintainers. Additionally Simple Sequence Repeat (SSR) markers were used to characterize 18 selected entries comprising 8 effective restorers, 4 partial restorers, 2 weak maintainers and 4 complete maintainers. Analysis of divergence patterns based on amplification profiles obtained from 34 SSR markers, allowed differentiation of effective fertility restorers from the rest of the entries comprising partial fertility restorers, weak sterility maintainers and complete sterility maintainers.
机译：狭窄的遗传基础和数量不足的父母线是特定于特异性杂交水稻品种的主要限制。我们研究了4种野生物质（WA）细胞质雄性无菌（CMS）线和45种不同的起源的花粉父母，以识别使用测试十字架的潜在恢复器和维护者。来自85个成功交叉的花粉活力测试能够将父母系分为恢复液，部分恢复器，部分维护者和维护者。另外，简单的序列重复（SSR）标记用于表征18个选定的条目，包括8个有效恢复液，4个部分恢复器，2个弱维护者和4个完整的维护者。基于从34个SSR标记获得的扩增谱的分解模式分析，允许从包含部分生育恢复器，弱不育系人维护者和完全无菌维护者的剩余条目中的有效生育恢复器的分化。

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