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首页> 外文期刊>International Journal of Electrochemical Science >Development of a Novel Electrochemical Monitoring Method of Enzymic Hydrolysis
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Development of a Novel Electrochemical Monitoring Method of Enzymic Hydrolysis

机译：一种新型酶水解电化学监测方法的研制

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In this work, we assessed the electrochemical behaviour of the products of substrates of the enzyme label, alkaline phosphate, ordinarily adopted into the field of electrochemical immunosensors. Cyclic voltammetry (CV) as well as amperometry of such resultants were respectively conducted at glassy carbon (GC) and gold (Au) electrodes. With mouse IgG to be a model, an ALP enzyme-magnified amperometric immunosensor with a sandwich shape came into being. Such immunosensor worked through the electropolymerization of o-aminobenzoic acid (o-ABA) polymer with conductivity on Au as well as GC electrodes’ appearance. Then the anti-mouse IgG adhered to the electrode appearance by covalent bonding between IgG antibody and the carboxyl species from poly(o-ABA). When 2- phospho-l-ascorbic acid was adopted to be a substrate, the most optimized signal could be generated through the poly(o-ABA)/Au immunosensor, indicating amperometric immunosensors with the basis of a conductive polymer electrode system were of great sensitivity to concentrations of the mouse IgG down to 1 ng/mL.
机译：在这项工作中，我们评估了酶标签碱基产品的电化学行为，碱性磷酸盐，通常采用电化学免疫调菌体领域。循环伏安法（CV）以及这种所得物的浓度测量分别在玻璃碳（GC）和金（Au）电极处进行。用小鼠IgG是一种模型，具有夹层形状的ALP酶放大的慢性免疫传感器。这种免疫传感器通过O-氨基苯甲酸（O-ABA）聚合物的电导聚合物而在AU和GC电极的外观上进行。然后通过来自聚（O-ABA）的IgG抗体和羧基物种之间的共价键合来粘附到电极外观上的抗小鼠IgG。当采用2-磷酸-1-抗坏血酸是基材时，可以通过聚（O-ABA）/ Au免疫传感器产生最优化的信号，指示具有导电聚合物电极系统的凹凸免疫传感器对小鼠IgG浓度的敏感性降至1ng / ml。

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