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首页> 外文期刊>International Journal of Environmental Research and Public Health >Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells
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Estrogenic Activity of Coumestrol, DDT, and TCDD in Human Cervical Cancer Cells

机译：CoumestroL，DDT和TCDD在人宫颈癌细胞中的雌激素活性

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Endogenous estrogens have dramatic and differential effects on classical endocrine organ and proliferation. Xenoestrogens are environmental estrogens that have endocrine impact, acting as both estrogen agonists and antagonists, but whose effects are not well characterized. In this investigation we sought to delineate effects of xenoestrogens. Using human cervical cancer cells (HeLa cells) as a model, the effects of representative xenoestrogens (Coumestrol-a phytoestrogen, tetrachlorodioxin (TCDD)-a herbicide and DDT-a pesticide) on proliferation, cell cycle, and apoptosis were examined. These xenoestrogens and estrogen inhibited the proliferation of Hela cells in a dose dependent manner from 20 to 120 nM suggesting, that 17-β-estrtadiol and xenoestrogens induced cytotoxic effects. Coumestrol produced accumulation of HeLa cells in G2/M phase, and subsequently induced apoptosis. Similar effects were observed in estrogen treated cells. These changes were associated with suppressed bcl-2 protein and augmented Cyclins A and D proteins. DDT and TCDD exposure did not induce apoptosis. These preliminary data taken together, suggest that xenoestrogens have direct, compound-specific effects on HeLa cells. This study further enhances our understanding of environmental modulation of cervical cancer.
机译：内源性雌激素对古典内分泌器官和增殖具有显着性和差异影响。卵黄素是具有内分泌撞击的环境雌激素，作为雌激素激动剂和拮抗剂，但其效果并不具备很好的表征。在这次调查中，我们寻求描绘卵雌激素的影响。使用人类宫颈癌细胞（HELA细胞）作为模型，研究了代表性异雌激素（COUMESTROL-A植物雌激素，四氯二氧化碳（TCDD）-A除草剂和DDT-A杀虫剂）对增殖，细胞周期和细胞凋亡的影响。这些卵黄素和雌激素抑制了20至120nm的剂量依赖性方式的HeLa细胞的增殖，提示，17-β-雌二醇和异雌激素诱导细胞毒性作用。 Coumestrol在G2 / M相中产生了HeLa细胞的积累，随后诱导细胞凋亡。在雌激素处理细胞中观察到类似的效果。这些变化与抑制的Bcl-2蛋白和增强的细胞周期蛋白A和D蛋白有关。 DDT和TCDD暴露没有诱导细胞凋亡。这些初步数据在一起，表明Xenoestrogens对HeLa细胞具有直接，复合的效果。本研究进一步增强了我们对宫颈癌环境调节的理解。

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