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首页> 外文期刊>African Journal of Microbiology Research >Occurrence and detection of AmpC -lactamases among some clinical isolates of Enterobacteriaceae obtained from Mansoura University Hospitals, Egypt
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Occurrence and detection of AmpC -lactamases among some clinical isolates of Enterobacteriaceae obtained from Mansoura University Hospitals, Egypt

机译：埃及曼苏拉大学医院获得的肠杆菌基肌的一些临床分离株中的AMPC - 酰胺酶的发生和检测

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摘要

The increasing incidence of β-lactam resistance due to AmpC β-lactamases in Egypt necessitated this study which aimed to evaluate four different phenotypic methods for detection of AmpC β-lactamases among some clinical isolates of Enterobacteriaceae and compare these results with those obtained using polymerase chain reaction. The distribution of five AmpC β-lactamases genes (AmpC,?CIT-M,?Fox-1,?ACC-1,?ACT-1) were determined among the clinical isolates. Among 180 clinical isolates of Enterobacteriaceae, only 57 isolates were AmpC producers by phenotypic methods and 108 were AmpC producers by polymerase chain reaction. Phenotypic methods adapted in this study gave variable results with the most discriminatory results given by direct inoculation of both the enzyme extract and the bacterial culture in the wells. Of these, the best results were given by enzyme inoculation methods where 43 isolates exhibited positive result by this method. The distribution of AmpC β-lactamases gene among the clinical isolates showed that?AmpC?gene predominated in?Escherichia coli.?Fox gene?was predominantly present in?Enterobacter cloacae?and?E. coli?isolates. ACT-1 predominated in?E. cloacae.?In contrast, enzymes from?CIT-M and ACC-1 group were rarely present in Enterobacteriaceae.
机译：由于埃及的AMPCβ-内酰胺酶导致的β-内酰胺抗酰胺酶的发生率越来越需要本研究，该研究旨在评估四种不同的表型方法，用于检测肠杆菌的一些临床分离株中的AMPCβ-内酰胺酶，并将这些结果与使用聚合酶链获得的那些结果进行比较反应。在临床分离株中确定了五个AMPCβ-内酰胺基因的分布（AMPC，β-内酰胺基因基因（AMPC，β-甲酰〜，福索-1，ΔACT-1）。在肠杆菌的180个临床分离物中，仅通过表型方法为AMPC生产商仅为AMPC生产商，通过聚合酶链反应108个是AMPC生产商。本研究适用的表型方法得到了可变的结果，通过直接接种孔中的酶提取物和细菌培养物给出的最多辨别结果。其中，通过酶接种方法给出了最佳结果，其中43分离物通过该方法表现出阳性结果。临床分离株中的AMPCβ-内酰胺基因的分布表明？AMPCα基因占主导地位的？富氏菌属基因，αfox基因αfr，α肠道粘附（肠菌）？和Δe。大肠杆菌？分离。 ACT-1主导于？e。 Cloacae.negasts，来自肠杆菌的酶，酶很少存在。

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