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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Occurrence and detection of AmpC β-lactamases among Enterobacteriaceae isolates from patients at Ain Shams University Hospital
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Occurrence and detection of AmpC β-lactamases among Enterobacteriaceae isolates from patients at Ain Shams University Hospital

机译：Ain Shams大学医院患者肠杆菌科分离株中AmpCβ-内酰胺酶的发生和检测

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Background AmpC β-lactamases are often plasmid mediated that hydrolyze all β-lactam antibiotics except cefepime and carbapenems. Aim of the study We aimed to evaluate the presence of AmpC β-lactamase among Enterobacteriaceae isolates separated from patients with nosocomial infections, and to detect the genetic basis for AmpC production in these strains. Subjects and methods 50 AmpC β-lactamase Enterobacteriaceae were analyzed for the presence of AmpC production. Three phenotypic AmpC confirmation assays (AmpC E test, the disk approximation test, Amp C EDTA disc) were able to detect the majority of AmpC-positive strains correctly. Molecular detection of plasmid mediated AmpC by multiplex PCR was conducted on them. Results The results show that from the 148 total isolates obtained from ICU admitted patients with suspected nosocomial infections, 50 (33.8%) were AmpC β-lactamase isolates. For the 50 AmpC isolates, all phenotypic Amp C tests gave a positive result. Among these isolates, plasmid encoded AmpC genes were detected by multiplex PCR in 46 (92%) isolates, which included Klebsiella pneumoniae ( n =22) (47.8%), Escherichia coli ( n =15) (32.6%), and Proteus mirabilis ( n =9) (19.6%). One E. coli , one K. pneumoniae , and two P. mirabilis isolates showed no AmpC gene. The most prevalent AmpC gene was that belonging to family CMY-1 which was detected in 73.9% (34/46) of all isolates. Conclusion It could be concluded that: Amp C producing isolates among Enterobacteriaceae strains have been increasingly recognized in the Ain Shams University Hospital. Thus, molecular identification of the genes encoding AmpC would be essential for a reliable epidemiological investigation of their transmission in hospitals.
机译：背景AmpCβ-内酰胺酶通常是质粒介导的，可水解除头孢吡肟和碳青霉烯外的所有β-内酰胺抗生素。研究的目的我们旨在评估与医院感染患者分离的肠杆菌科细菌中AmpCβ-内酰胺酶的存在，并检测这些菌株中AmpC产生的遗传基础。对象和方法分析了50个AmpCβ-内酰胺酶肠杆菌科细菌是否存在AmpC产生。三种表型AmpC确认测定（AmpC E测试，圆盘近似测试，Amp C EDTA圆盘）能够正确检测出大多数AmpC阳性菌株。通过多重PCR对质粒介导的AmpC进行分子检测。结果结果显示，在从ICU入院的疑似医院感染患者中获得的148株分离株中，有50株（33.8％）是AmpCβ-内酰胺酶分离株。对于50个AmpC分离株，所有表型Amp C测试均给出阳性结果。在这些分离株中，通过多重PCR在46个分离株（92％）中检测到质粒编码的AmpC基因，其中包括肺炎克雷伯菌（n = 22）（47.8％），大肠杆菌（n = 15）（32.6％）和变形杆菌（Proteus mirabilis） （n = 9）（19.6％）。 1株大肠杆菌，1株肺炎克雷伯菌和2株奇异疟原虫分离株均未显示AmpC基因。最流行的AmpC基因是属于CMY-1家族的基因，在所有分离株中占73.9％（34/46）。结论可以得出以下结论：在Ain Shams大学医院，肠杆菌科菌株中产生Amp C的分离株已得到越来越多的认可。因此，对AmpC编码基因的分子鉴定对于对其在医院中的传播进行可靠的流行病学调查至关重要。

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