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Transcriptogenomics identification and characterization of RNA editing sites in human primary monocytes using high-depth next generation sequencing data

机译：使用高深度下一代测序数据鉴定和表征人初级单核细胞中RNA编辑位点的鉴定及表征

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摘要

High-depth next generation sequencing data provide valuable insights into the number and distribution of RNA editing events. Here, we report the RNA editing events at cellular level of human primary monocyte using high-depth whole genomic and transcriptomic sequencing data. We identified over a ten thousand putative RNA editing sites and 69% of the sites were A-to-I editing sites. The sites enriched in repetitive sequences and intronic regions. High-depth sequencing datasets revealed that 90% of the canonical sites were edited at lower frequencies (0.7). Single and multiple human monocytes and brain tissues samples were analyzed through genome sequence independent approach. The later approach was observed to identify more editing sites. Monocytes was observed to contain more C-to-U editing sites compared to brain tissues. Our results establish comparable pipeline that can address current limitations as well as demonstrate the potential for highly sensitive detection of RNA editing events in single cell type.
机译：高深下一代测序数据为RNA编辑事件的数量和分布提供了有价值的见解。在这里，我们使用高深整个基因组和转录组测序数据报告人初级单核细胞细胞水平的RNA编辑事件。我们确定了一万次推定的RNA编辑网站，69％的网站是A-to-i编辑网站。富集的网站富集序列和内文区域。高深测序数据集显示，90％的规范位点在较低频率下进行编辑（<0.7）。通过基因组序列独立方法分析单胞和多种人单核细胞和脑组织样品。观察到后来的方法来识别更多的编辑网站。观察到单核细胞与脑组织相比含有更多的C-u对U编辑位点。我们的结果建立了可比的管道，可以解决当前限制，并证明单细胞类型中RNA编辑事件的高度敏感性检测的可能性。

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