• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >Applying microarray-based technique to study and analyze silkworm (Bombyx mori) transcriptomic response to long-term high iron diet
【24h】

Applying microarray-based technique to study and analyze silkworm (Bombyx mori) transcriptomic response to long-term high iron diet

机译：应用基于微阵列的技术研究和分析蚕（BOMBX MORI）转录组对长期高铁饮食的反应

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To investigate the biological processes affected by long-term iron supplementation, newly hatched silkworms were exposed to high iron mulberry diet (10 and 100?ppm) and its effect on silkworm transcriptom was determined. The results showed that the silkworm was responsive to iron by increasing iron concentration and ferritin levels in the hemolymph and by regulating the expression of many other genes. A total of 523 and 326 differentially expressed genes were identified in 10 and 100?ppm Fe group compared to the control, respectively. Of these genes, 249 were shared between in both the 10?ppm and 100?ppm Fe group, including 152 up-regulated and 97 down-regulated genes. These shared genes included 19 known Fe regulated, 24 immune-related, 12 serine proteases and serine proteases homologs, 41 cuticular and cuticle genes. Ten genes (carboxypeptidases A, serine protease homologs 85, fibrohexamerin/P25, transferrin, sex-specific storage-protein 2, fungal protease inhibitor F, insect intestinal mucin, peptidoglycan recognition protein B, cuticle protein CPH45, unknown gene) were involved in the regulation of iron overload responses.
机译：为了探讨受长期铁补充影响的生物过程，新孵化的蚕均暴露于高铁桑椹饮食（10和100〜ppm），测定其对蚕转录组的影响。结果表明，通过增加血淋巴中的铁浓度和铁蛋白水平并通过调节许多其他基因的表达，蚕对铁响应。与对照相比，总共523和326次差异表达基因分别鉴定在10和100μlPPMFe组中。在这些基因中，在10·ppm和100·ppm Fe组中共用249，其中包括152个上调和97个下调基因。这些共享基因包括19个已知的Fe调节，24个免疫相关，12种丝氨酸蛋白酶和丝氨酸蛋白质同源物，41个点心和角质质基因。 10基因（羧肽酶A，丝氨酸蛋白质同源物85，纤维酚素/ p25，转移素，性别特异性储存 - 蛋白2，真菌蛋白酶抑制剂F，昆虫肠粘蛋白，肽蛋白识别蛋白B，角质蛋白CPH45，未知基因）涉及铁过载响应的调节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号