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首页> 外文期刊>Genomics >Applying microarray-based technique to study and analyze silkworm (Bombyx mori) transcriptomic response to long-term high iron diet
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Applying microarray-based technique to study and analyze silkworm (Bombyx mori) transcriptomic response to long-term high iron diet

机译：应用基于微阵列的技术研究和分析蚕（Bombyx Mori）转录组的长期高铁饮食的反应

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摘要

To investigate the biological processes affected by long-term iron supplementation, newly hatched silkworms were exposed to high iron mulberry diet (10 and 100 ppm) and its effect on silkworm transcriptom was determined. The results showed that the silkworm was responsive to iron by increasing iron concentration and ferritin levels in the hemolymph and by regulating the expression of many other genes. A total of 523 and 326 differentially expressed genes were identified in 10 and 100 ppm Fe group compared to the control, respectively. Of these genes, 249 were shared between in both the 10 ppm and 100 ppm Fe group, including 152 up-regulated and 97 down-regulated genes. These shared genes included 19 known Fe regulated, 24 immune-related, 12 serine proteases and serine proteases homologs, 41 cuticular and cuticle genes. Ten genes (carboxypeptidases A, serine protease homologs 85, fibrohexamerin/P25, transferrin, sex-specific storage-protein 2, fungal protease inhibitor F, insect intestinal mucin, peptidoglycan recognition protein B, cuticle protein CPH45, unknown gene) were involved in the regulation of iron overload responses.
机译：为了研究受长期铁补充影响的生物学过程，将新孵化的蚕均暴露在高铁桑椹饮食（10和100ppm）上，测定其对蚕转录组的影响。结果表明，通过增加血淋巴中的铁浓度和铁蛋白水平并通过调节许多其他基因的表达，蚕对铁响应。与对照分别在10和100ppm Fe组中鉴定了总共523和326个差异表达基因。在这些基因中，在10ppm和100ppm Fe组中共有249个，其中包括152个上调和97个下调基因。这些共享基因包括19个已知的Fe调节，24个免疫相关，12种丝氨酸蛋白酶和丝氨酸蛋白质同源物，41个点心和角质质基因。十个基因（羧肽酶A，丝氨酸蛋白质同源物85，纤维酚素/ p25，转移素，性别特异性储存 - 蛋白2，真菌蛋白酶抑制剂F，昆虫肠粘蛋白，肽聚糖识别蛋白B，角质蛋白CPH45，未知基因）均涉及铁超载响应的调节。

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