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首页> 外文期刊>Genomics >Identification, characterization, and expressions profile analysis of growth hormone receptors (GHR1 and GHR2) in Hybrid grouper (Epinephelus fuscoguttatus ♀?×?Epinephelus polyphekadion ♂)
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Identification, characterization, and expressions profile analysis of growth hormone receptors (GHR1 and GHR2) in Hybrid grouper (Epinephelus fuscoguttatus ♀?×?Epinephelus polyphekadion ♂)

机译：杂交物格鲁普尔（Epinephelus fuscoguttatus♀β×β×β×x≤Polyphekion♂）的生长激素受体（GHR1和GHR2）的鉴定，表征和表达概况分析

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摘要

Growth hormone is an essential hormone that plays essential roles in growth, metabolism, cellular differentiation, immunity and reproduction in fish, by means of the growth hormone receptors. The encoding cDNA growth hormone receptors (GHR1 and GHR2) were cloned and characterized from Hybrid grouper (Epinephelus fuscoguttatus♀?×?Epinephelus polyphekadion♂). Sequence analysis of the cloned GHR1 was observed as containing 2176, which comprised an ORF of 1842?bp, 5 UTR of 6?bp and 3 UTR of 328?bp, with 612 amino acids encoding proteins, while GHR2 was observed as containing 1824?bp that encompassed an ORF of 708?bp, 5 UTR of 48?bp and 3 UTR of 1068?bp with 235 amino acids encoding proteins. Relative mRNA expression of GHR1 and GHR2 in the liver and muscle was found to be highest respectively. Our findings provide vital statistics of GHRs likely to play a significant role in the growth of the fish.
机译：生长激素是通过生长激素受体在鱼类中发挥生长，代谢，细胞分化，免疫和繁殖的基本作用的基本激素。克隆编码cDNA生长激素受体（GHR1和GHR2）并用杂交物格鲁珀（EPINEPHELUSFUSCOGUTTATUSα）的特征为例α×β-肾盂pOMPHEKADION）。观察到克隆的GHR1的序列分析，如含有2176，其包含1842μl的ORF，5 utr为6·bp和328 utr的3 utr 328？Bp，编码蛋白的612个氨基酸，而GHR2被观察到含有1824℃的氨基酸。 BP包含708磅的ORF，5 utr为48磅的48磅，3 utr为1068 utr为1068μlβbp，其具有235个氨基酸编码蛋白质。发现肝脏和肌肉中GHR1和GHR2的相对mRNA表达分别最高。我们的调查结果提供了GHR的重要统计，可能在鱼类的生长中发挥重要作用。

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