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Transcriptomic analyses of gene expression by CRISPR knockout of miR-214 in cervical cancer cells

机译：宫颈癌细胞MiR-214 Crispr敲除基因表达的转录组分析

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In this study, we investigate the effect of one such micro RNA, miR-214 which is frequently down-regulated in cervical cancer. In this study, we either CRISPR knocked out or overexpressed miR-214 in cervical cancer cells and analyzed the global mRNA expression by Next Generation Sequencing (NGS) It was observed that a total of 108 genes were upregulated and 178 downregulated between the samples, above and below the baseline respectively. Gene Ontology and KEGG pathway analysis reveal distinct biological processes and pathways. Analysis of gene regulatory networks also gave different network patterns in the two samples. We confirmed the RNA sequencing data for 10 genes; IFIF27, SMAD3, COX11, TP53INP1, ABL2, FGF8, TNFAIP3, NRG1, SP3 and MDM4 by Real-time PCR. This is the first report on the effect of miR-214 on global mRNA profile in cervical cancer cells. This study also reports new biomarkers for cervical cancer prognosis.
机译：在这项研究中，我们研究了一种如此微RNA，miR-214在宫颈癌中经常下调的效果。在这项研究中，我们要么在宫颈癌细胞中撞出或过表达miR-214，并通过下一个产生的序列（NGS）分析了全局mRNA表达，观察到总共108个基因在上面的样品之间进行了上调，178个下调分别在基线下方。基因本体和Kegg途径分析显示不同的生物过程和途径。基因监管网络的分析也给出了两个样品中的不同网络模式。我们确认了10个基因的RNA测序数据; IFIF27，SMAD3，COX11，TP53INP1，ABL2，FGF8，TNFAIP3，NRG1，SP3和MDM4通过实时PCR。这是关于miR-214对宫颈癌细胞全球mRNA谱效应的第一报告。本研究还报告了宫颈癌预后的新生物标志物。

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