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Prepupae and pupae transcriptomic characterization of Trichogramma chilonis

机译：prepupae和pupae trichogramma chilonis的转录组特征

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The egg parasitoid, Trichogramma chilonis, has significant control effects on agriculture and forestry pests and is widely employed in southern China for the biological control of lepidopteran pests. In this study, transcriptomic analysis was used to gain a clear understanding of the molecular changes in prepupae and pupae of T. chilonis. A total of 16.88 Gb of clean data were obtained and finally assembled into 43,136 unigenes, 18,880 of which were annotated. After FPKM standardization, 117 and 838 specific expression genes were found in prepupae and pupae, respectively. There were 3129 differentially expressed genes between prepupae and pupae. Compared to pupae, 806 genes were up-regulated and 2323 were down-regulated in prepupae. Background on the T. chilonis transcriptome, the enriched GO function and KEGG pathway analysis of DEGs were considered. As indicated by GO classification, up-regulated genes were mainly involved in chitin metabolism, cell adhesion and endocytic, while most down-regulated genes were involved in synthesis of cell components, ion transport and biological regulation. KEGG enrichment analysis showed that 458 DEGs were enriched in 94 metabolic pathways. DEGs involved in nucleotide replication and transcription, substance metabolism, insect hormone biosynthesis, cell growth and death, reproductive metabolism, circadian rhythms and signal transduction pathways were up-regulated or down-regulated to different degrees, indicating that these genes played important roles during the process of metamorphosis in T. chilonis. This study provides a rich data source for the future study of T. chilonis molecular and biological mechanisms. A large number of genes related to metamorphosis were found based on comparison analysis between prepupae and pupae transcriptomes. This study lays a good foundation for in-depth study of gene transcription and regulation mechanisms during T. chilonis metamorphosis.
机译：蛋寄生蛋白剧烈地区嗜睡，对农业和林业有重大的控制影响，在中国南部广泛采用鳞翅目害虫的生物控制。在这项研究中，通过转录组分析用于清楚地了解辣ONIS的Prepupae和Pupae的分子变化。获得总共16.88GB的清洁数据，最终组装成43,136个unigenes，其中18,880个被注释。在FPKM标准化之后，分别在Prepupae和Pupae中发现117和838个特异性表达基因。 Prepupae和Pupae之间存在3129个差异表达基因。与PUPAE相比，上调806个基因，预备2323个受调节。背景技术在T.Chilonis转录组中，考虑了富集的GO功能和DEGS的KEGG途径分析。如去分类所示，上调基因主要参与几丁质代谢，细胞粘附和内吞，而大多数下调基因涉及细胞成分，离子转运和生物调节的合成。 Kegg富集分析表明，94种代谢途径富集了458次。参与核苷酸复制和转录，物质代谢，昆虫激素生物合成，细胞生长和死亡，生殖代谢，昼夜节律和信号转导途径的次数被上调或下调到不同程度，表明这些基因在此期间起到重要作用T.Chilonis的变态过程。本研究为未来的T.Chilonis分子和生物机制提供了丰富的数据源。基于Prepupae和Pupae转录om之间的比较分析，发现了大量与变态相关的基因。本研究为在T.Chilonis变态期间对基因转录和调节机制进行了深入研究的良好基础。

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