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首页> 外文期刊>Genomics >Skin transcriptome profiles associated with black- and white-coated regions in Boer and Macheng black crossbred goats
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Skin transcriptome profiles associated with black- and white-coated regions in Boer and Macheng black crossbred goats

机译：皮肤转录组谱与布尔和町黑色杂交山羊的黑色和白色涂层地区相关联

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To increase the current understanding of the gene-expression profiles in different skin regions associated with different coat colors and identify key genes for the regulation of color patterns in goats, we used the Illumina RNA-Seq method to compare the skin transcriptomes of the black- and white-coated regions containing hair follicles from the Boer and Macheng Black crossbred goat, which has a black head and a white body. Six cDNA libraries derived from skin samples of the white-coated region (n?=?3) and black-coated region (n?=?3) were constructed from three full-sib goats. On average, we obtained approximately 76.5 and 73.5 million reads for skin samples from black- and white-coated regions, respectively, of which 75.39% and 76.05% were covered in the genome database. A total of 165 differentially expressed genes (DEGs) were detected between these two color regions, among which 110 were upregulated and 55 were downregulated in the skin samples of white- vs. black-coated regions. The results of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that some of these DEGs may play an important role in controlling the pigmentation of skin or hair follicles. We identified three key DEGs, i.e., Agouti, DCT, and TYRP1, in the pathway related to melanogenesis in the different skin regions of the crossbred goat. DCT and TYRP1 were downregulated and Agouti was upregulated in the skin of the white-coated region, suggesting a lack of mature melanocytes in this region and that Agouti might play a key developmental role in color-pattern formation. All data sets (Gene Expression Omnibus) are available via public repositories. In addition, MC1R was genotyped in 200 crossbred goats with a black head and neck. Loss-of-function mutations in MC1R as well as homozygosity for the mutant alleles were widely found in this population. The MC1R gene did not seem to play a major role in determining the black head and neck in our crossbred goats. Our study provides insights into the transcriptional regulation of two distinct coat colors, which might serve as a key resource for understanding coat color pigmentation in goats. The region-specific expression of Agouti may be associated with the distribution of pigments across the body in Boer and Macheng Black crossbred goats.
机译：为了增加与不同涂层颜色相关的不同皮肤区域的基因表达谱的目前的理解，并鉴定山羊中颜色模式调节的关键基因，我们使用了Illumina RNA-SEQ方法来比较黑色的皮肤转录组和含有波尔和町黑杂交山羊的毛囊的白色涂层区域，具有黑头和白色的身体。从三个全SIB山羊构建了六种来自白涂层区域（n =Δ3）和黑涂层区域（n≤=Δ3）的皮肤样品的六个cDNA文库。平均而言，我们分别获得了大约76.5％和73.5百万的皮肤样品，分别是来自黑色和白涂层区域的皮肤样品，其中在基因组数据库中涵盖了75.39％和76.05％。在这两个颜色区域之间检测到总共165个差异表达基因（DEG），其中上调110，在黑涂层区域的皮肤样品中下调55个。基因本体论和基因植物的京都植物和基因组富集分析结果表明，其中一些可在控制皮肤或毛囊的色素沉着中起着重要作用。在杂交山羊的不同皮肤区域中，我们确定了三个关键的DEG，即Agouti，DCT和TyrP1，在与杂色地区的不同皮肤区域相关的途径中。 DCT和TyrP1被下调，刺尾术在白涂层地区的皮肤上上调，这表明该地区缺乏成熟的黑素细胞，而且agouti可能在颜色模式形成中发挥关键发展作用。所有数据集（基因表达式omnibus）可通过公共存储库获得。此外，MC1R在200个杂交山羊的基因分型，带有黑头和颈部。在该群群中广泛发现MC1R中的功能突变以及突变等位基因的纯合子。 MC1R基因似乎并未在确定我们杂交山羊中的黑头和颈部中发挥重要作用。我们的研究提供了两种不同涂层颜色的转录调控的见解，这可能是理解山羊涂色颜色色素沉着的关键资源。 Agouti的特异性特异性表达可能与Boer和Macheng黑色杂交山羊穿过身体的颜料的分布相关。

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