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首页> 外文期刊>Genomics >Knockdown of Gh_A05G1554 (GhDHN_03) and Gh_D05G1729 (GhDHN_04) Dehydrin genes, Reveals their potential role in enhancing osmotic and salt tolerance in cotton
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Knockdown of Gh_A05G1554 (GhDHN_03) and Gh_D05G1729 (GhDHN_04) Dehydrin genes, Reveals their potential role in enhancing osmotic and salt tolerance in cotton

机译：GH_A05G1554（GHDHN_03）和GH_D05G1729（GHDHN_04）脱氢基因的敲低揭示了它们在增强棉花中渗透和耐盐性方面的潜在作用

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In this investigation, whole-genome identification and functional characterization of the cotton dehydrin genes was carried out. A total of 16, 7, and 7 dehydrin proteins were identified in G. hirsutum, G. arboreum and G. raimondii, respectively. Through RNA sequence data and RT-qPCR validation, Gh_A05G1554 (GhDHN_03) and Gh_D05G1729 (GhDHN_04) were highly upregulated, and knockdown of the two genes, significantly reduced the ability of the plants to tolerate the effects of osmotic and salt stress. The VIGS-plants recorded significantly higher concentration levels of oxidants, hydrogen peroxide (H2O2) and malondialdehyde (MDA), furthermore, the four stress responsive genes GhLEA2, Gh_D12G2017 (CDKF4), Gh_A07G0747 (GPCR) and a transcription factor, trihelix, Gh_A05G2067, were significantly downregulated in VIGS-plants, but upregulated in wild types under osmotic and salt stress condition. The result indicated that dehydrin proteins are vital for plants and can be exploited in developing a more osmotic and salt stress-resilient germplasm to boost and improve cotton production.
机译：在该研究中，进行了棉脱氢基因的全基因组鉴定和功能性表征。在G.Hirsutum，G. arboreum和G. Raimondii中鉴定了总共16,7和7和7个脱氢蛋白。通过RNA序列数据和RT-QPCR验证，高度上调GH_A05G1554（GHDHN_03）和GH_D05G1729（GHDHN_04）和两个基因的敲低，显着降低了植物侵蚀渗透和盐胁迫的影响的能力。 Vigs-植物记录了较高的浓度浓度较高的氧化剂，过氧化氢（H 2 O 2）和丙二醛（MDA），此外，四个应力响应基因GHLEA 2，GH_D12G2017（CDKF4），GH_A07G0747（GPCR）和转录因子，Trihelix，GH_A05G2067，在Vigs-植物中显着下调，但在渗透和盐胁迫条件下在野生类型中令人越纪调整。结果表明，脱氢蛋白对植物至关重要，可以在开发更渗透和盐胁迫 - 弹性种质中以提高和改善棉花生产。

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