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首页> 外文期刊>Genomics >Characterization of two kcnk3 genes in Nile tilapia (Oreochromis niloticus): Molecular cloning, tissue distribution, and transcriptional changes in various salinity of seawater
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Characterization of two kcnk3 genes in Nile tilapia (Oreochromis niloticus): Molecular cloning, tissue distribution, and transcriptional changes in various salinity of seawater

机译：尼罗亚替尼亚（OREOCHROMIS NILORICUS）中两种KCNK3基因的特征：海水各种盐度的分子克隆，组织分布和转录变化

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As one important member of the two-pore-domain potassium channel (K2P) family, potassium channel subfamily K member 3 (KCNK3) has been reported for thermogenesis regulation, energy homeostasis, membrane potential conduction, and pulmonary hypertension in mammals. However, its roles in fishes are far less examined and published. In the present study, we identified two kcnk3 genes (kcnk3a and kcnk3b) in an euryhaline fish, Nile tilapia (Oreochromis niloticus), by molecular cloning, genomic survey and laboratory experiments to investigate their potential roles for osmoregulation. We obtained full-length coding sequences of the kcnk3a and kcnk3b genes (1209 and 1173?bp), which encode 402 and 390 amino acids, respectively. Subsequent multiple sequence alignments, putative 3D-structure model prediction, genomic survey and phylogenetic analysis confirmed that two kcnk3 paralogs are widely presented in fish genomes. Interestingly, a DNA fragment inversion of a kcnk3a cluster was found in Cypriniforme in comparison with other fishes. Quantitative real-time PCRs demonstrated that both the tilapia kcnk3 genes were detected in all the examined tissues with a similar distribution pattern, and the highest transcriptions were observed in the heart. Meanwhile, both kcnk3 genes in the gill were proved to have a similar transcriptional change pattern in response to various salinity of seawater, implying that they might be involved in osmoregulation. Furthermore, three predicted transcription factors (arid3a, arid3b, and arid5a) of both kcnk3 genes also showed a similar pattern as their target genes in response to the various salinity, suggesting their potential positive regulatory roles. In summary, we for the first time characterized the two kcnk3 genes in Nile tilapia, and demonstrated their potential involvement in osmoregulation for this economically important fish.
机译：作为双孔径域钾通道（K2P）家族的一个重要成分，据报道了钾通道亚家族K成员3（KCNK3）用于哺乳动物的热生成调节，能量稳态，膜电位传导和肺动脉高压。然而，它在鱼类中的角色远远不那么审查和发表。在本研究中，通过分子克隆，基因组调查和实验室实验，在肠青鱼，尼罗河罗非鱼（Oreochromis Niloticus）中鉴定了两种KCNK3基因（KCNK3A和KCNK3B），以研究其渗透渗透的潜在作用。我们获得了KCNK3A和KCNK3B基因（1209和1173μlBP）的全长编码序列，其分别编码402和390个氨基酸。随后的多个序列比对，推定的3D结构模型预测，基因组测量和系统发育分析证实，两种KCNK3旁系蛋白酶广泛呈现在鱼类基因组中。有趣的是，与其他鱼类相比，在Cypriniforme中发现了KCNK3A簇的DNA片段反转。定量实时PCR表明，在所有相关的分布图案中检测到罗非鱼KCNK3基因，并且在心脏中观察到最高的转录。同时，证明鳃中的KCNK3基因均响应海水的各种盐度具有类似的转录变化模式，这意味着它们可能参与OSMoreGulation。此外，KCNK3基因的三种预测转录因子（ARID3A，ARID3B和ARID5A）也表现出与其靶基因相似的模式，响应于各种盐度，表明其潜在的阳性调节作用。总之，我们首次表现了尼罗罗非鱼的两种KCNK3基因，并证明了它们对这种经济上重要的鱼类的渗透渗透性。

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