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首页> 外文期刊>Genomics >De novo assembly transcriptome analysis reveals the preliminary molecular mechanism of pigmentation in juveniles of the hard clam Mercenaria mercenaria
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De novo assembly transcriptome analysis reveals the preliminary molecular mechanism of pigmentation in juveniles of the hard clam Mercenaria mercenaria

机译：De Novo组装转录组分析显示了硬蛤梅纳里亚群体幼虫幼虫中色素沉着的初步分子机制

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摘要

Color plays a vital function in camouflage, sexual selection, immunity, and evolution. Mollusca possess vivid shell colors and pigmentation starts at the juvenile stage. The hard clam Mercenaria mercenaria is a widely cultivated bivalve of high economic value. To explore the molecular mechanism of pigmentation in juvenile clams, here, we performed RNA-Seq analysis on non-pigmented, white, and red M. mercenaria specimens. Clean reads were assembled into 358,285 transcripts and 149,234 unigenes, whose N50 lengths were 2107?bp and 1567?bp, respectively. Differentially expressed genes were identified and analyzed for KEGG enrichment. “Melanoma/Melanogenesis”, “ABC transporters”, and “Porphyrin and chlorophyll metabolism” pathways appeared to be associated with pigmentation. Pathways related to carotenoid metabolism seemed to also play a vital role in pigmentation in juveniles. Our results provide new insights into the formation of shell color in juvenile hard clams.
机译：颜色在伪装，性选择，免疫力和演变中起着重要功能。软体动物具有生动的壳体颜色，色素沉着在少年阶段开始。硬蛤蜊Mercenaria Mercenaria是一种广泛栽培的高经济价值。为了探讨少年蛤的色素沉着的分子机制，在这里，我们对非着色，白色和红绿鳞片标本进行了RNA-SEQ分析。将清洁的读数组装成358,285份转录物和149,234个unigenes，其N50长度分别为2107？BP和1567磅BP。鉴定差异表达基因并分析了Kegg富集。 “黑素瘤/黑色素生成”，“ABC转运蛋白”和“卟啉和叶绿素代谢”途径似乎与色素沉着相关。与类胡萝卜素代谢相关的途径似乎也在青少年中的色素沉着中起着至关重要的作用。我们的结果为少年硬蛤中壳颜色的形成提供了新的见解。

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